Cells exhibiting strong p16 INK4a promoter activation in vivo display features of senescence.

Autor:	Liu JY; Curriculum in Genetics and Molecular Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599.; The Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Souroullas GP; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110., Diekman BO; The Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599.; Thurston Arthritis Research Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599.; Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 and, North Carolina State University, Raleigh, NC 27695., Krishnamurthy J; The Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Hall BM; Research Division, Everon Biosciences, Inc., Buffalo, NY 14203., Sorrentino JA; The Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Parker JS; The Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599.; Department of Genetics, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Sessions GA; Thurston Arthritis Research Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599., Gudkov AV; Research Division, Everon Biosciences, Inc., Buffalo, NY 14203.; Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263., Sharpless NE; Curriculum in Genetics and Molecular Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599; norman.sharpless@nih.gov.; The Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599.; Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC 27599.; Office of the Director, The National Cancer Institute, Bethesda, MD 20892.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Feb 12; Vol. 116 (7), pp. 2603-2611. Date of Electronic Publication: 2019 Jan 25.
DOI:	10.1073/pnas.1818313116
Abstrakt:	The activation of cellular senescence throughout the lifespan promotes tumor suppression, whereas the persistence of senescent cells contributes to aspects of aging. This theory has been limited, however, by an inability to identify and isolate individual senescent cells within an intact organism. Toward that end, we generated a murine reporter strain by "knocking-in" a fluorochrome, tandem-dimer Tomato (tdTom), into exon 1α of the p16 INK4a locus. We used this allele ( p16 tdTom ) for the enumeration, isolation, and characterization of individual p16 INK4a -expressing cells (tdTom + ). The half-life of the knocked-in transcript was shorter than that of the endogenous p16 INK4a mRNA, and therefore reporter expression better correlated with p16 INK4a promoter activation than p16 INK4a transcript abundance. The frequency of tdTom + cells increased with serial passage in cultured murine embryo fibroblasts from p16 tdTom/+ mice. In adult mice, tdTom + cells could be readily detected at low frequency in many tissues, and the frequency of these cells increased with aging. Using an in vivo model of peritoneal inflammation, we compared the phenotype of cells with or without activation of p16 INK4a and found that tdTom + macrophages exhibited some features of senescence, including reduced proliferation, senescence-associated β-galactosidase (SA-β-gal) activation, and increased mRNA expression of a subset of transcripts encoding factors involved in SA-secretory phenotype (SASP). These results indicate that cells harboring activation of the p16 INK4a promoter accumulate with aging and inflammation in vivo, and display characteristics of senescence. Competing Interests: The authors declare no conflict of interest. (Copyright © 2019 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
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