Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies.

Autor:	González-Bermúdez B; Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, Spain; Departamento de Ciencia de Materiales, ETSI de Caminos, Canales y Puertos, Universidad Politécnica de Madrid, Madrid, Spain., Guinea GV; Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, Spain; Departamento de Ciencia de Materiales, ETSI de Caminos, Canales y Puertos, Universidad Politécnica de Madrid, Madrid, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain., Plaza GR; Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, Spain; Departamento de Ciencia de Materiales, ETSI de Caminos, Canales y Puertos, Universidad Politécnica de Madrid, Madrid, Spain. Electronic address: gustavo.plaza@upm.es.
Jazyk:	angličtina
Zdroj:	Biophysical journal [Biophys J] 2019 Feb 19; Vol. 116 (4), pp. 587-594. Date of Electronic Publication: 2019 Jan 07.
DOI:	10.1016/j.bpj.2019.01.004
Abstrakt:	With five decades of sustained application, micropipette aspiration has enabled a wide range of biomechanical studies in the field of cell mechanics. Here, we provide an update on the use of the technique, with a focus on recent developments in the analysis of the experiments, innovative microaspiration-based approaches, and applications in a broad variety of cell types. We first recapitulate experimental variations of the technique. We then discuss analysis models focusing on important limitations of widely used biomechanical models, which underpin the urge to adopt the appropriate ones to avoid misleading conclusions. The possibilities of performing different studies on the same cell are also considered. (Copyright © 2019 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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