Characterization of Xenopus laevis guanine deaminase reveals new insights for its expression and function in the embryonic kidney.

Autor: Slater PG; Boston College, Department of Biology, Chestnut Hill, Massachusetts., Cammarata GM; Boston College, Department of Biology, Chestnut Hill, Massachusetts., Monahan C; Boston College, Department of Biology, Chestnut Hill, Massachusetts., Bowers JT; Boston College, Department of Biology, Chestnut Hill, Massachusetts., Yan O; Boston College, Department of Biology, Chestnut Hill, Massachusetts., Lee S; Boston College, Department of Biology, Chestnut Hill, Massachusetts., Lowery LA; Boston College, Department of Biology, Chestnut Hill, Massachusetts.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2019 Apr; Vol. 248 (4), pp. 296-305. Date of Electronic Publication: 2019 Feb 19.
DOI: 10.1002/dvdy.14
Abstrakt: Background: The mammalian guanine deaminase (GDA), called cypin, is important for proper neural development, by regulating dendritic arborization through modulation of microtubule (MT) dynamics. Additionally, cypin can promote MT assembly in vitro. However, it has never been tested whether cypin (or other GDA orthologs) binds to MTs or modulates MT dynamics. Here, we address these questions and characterize Xenopus laevis GDA (Gda) for the first time during embryonic development.
Results: We find that exogenously expressed human cypin and Gda both display a cytosolic distribution in primary embryonic cells. Furthermore, while expression of human cypin can promote MT polymerization, Xenopus Gda has no effect. Additionally, we find that the tubulin-binding collapsin response mediator protein (CRMP) homology domain is only partially conserved between cypin and Gda. This likely explains the divergence in function, as we discovered that the cypin region containing the CRMP homology and PDZ-binding domain is necessary for regulating MT dynamics. Finally, we observed that gda is strongly expressed in the kidneys during late embryonic development, although it does not appear to be critical for kidney development.
Conclusions: Together, these results suggest that GDA has diverged in function between mammals and amphibians, and that mammalian GDA plays an indirect role in regulating MT dynamics. Developmental Dynamics 248:296-305, 2019. © 2019 Wiley Periodicals, Inc.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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