Lactobacillus reuteri DSM 17938 Protects against Gastric Damage Induced by Ethanol Administration in Mice: Role of TRPV1/Substance P Axis.

Autor:	Oliveira AP; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil. apatriciabiomed@gmail.com., Souza LKM; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil. luankelves11@gmail.com., Araújo TSL; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil. thiago_parnaiba@hotmail.com., Araújo S; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil. simonearaujoufpi@gmail.com., Nogueira KM; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil. keerolayne@hotmail.com., Sousa FBM; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil. biatrix14@hotmail.com., Silva RO; Department of Physiology and Pharmacology, Federal University of Ceará, CEP 60430-270, Fortaleza, Ceará, Brazil. renan.oliveira25@yahoo.com.br., Pacífico DM; Postgraduate Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University Ceará, CEP 60430-170, Fortaleza-CE, Brazil. dvisonpacifico@gmail.com., Martins CS; Postgraduate Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University Ceará, CEP 60430-170, Fortaleza-CE, Brazil. concmartins@hotmail.com., Brito GAC; Postgraduate Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University Ceará, CEP 60430-170, Fortaleza-CE, Brazil. gerlyanneb@hotmail.com., Souza MHLP; Department of Physiology and Pharmacology, Federal University of Ceará, CEP 60430-270, Fortaleza, Ceará, Brazil. souzamar@hotmail.com., Medeiros JVR; Laboratory of Pharmacology of Inflammation and Gastrointestinal Disorders (Lafidg), Federal University of Piauí, Av. São Sebastião, nº 2819, CEP 64202-02, Parnaíba, PI, Brazil. jandvenes@ufpi.edu.br.
Jazyk:	angličtina
Zdroj:	Nutrients [Nutrients] 2019 Jan 21; Vol. 11 (1). Date of Electronic Publication: 2019 Jan 21.
DOI:	10.3390/nu11010208
Abstrakt:	This study aimed to evaluate the effect of Lactobacillus reuteri DSM 17938 (DSM) on ethanol-induced gastric injury, and if its possible mechanism of action is related to inhibiting the transient receptor potential vanilloid type 1 (TRPV1). We evaluated the effect of supplementing 10⁸ CFU•g body wt -1 •day -1 of DSM on ethanol-induced gastric injury. DSM significantly reduced the ulcer area (1.940 ± 1.121 mm²) with 3 days of pretreatment. The effects of DSM supplementation were reversed by Resiniferatoxin (RTX), TRPV1 agonist (3 nmol/kg p.o.). Substance P (SP) (1 μmol/L per 20 g) plus 50% ethanol resulted in hemorrhagic lesions, and DSM supplementation did not reverse the lesion area induced by administering SP. TRPV1 staining intensity was lower, SP, malondialdehyde (MDA) and nitrite levels were reduced, and restored normal levels of antioxidant parameters (glutathione and superoxide dismutase) in the gastric mucosa in mice treated with DSM. In conclusion, DSM exhibited gastroprotective activity through decreased expression of TRPV1 receptor and decreasing SP levels, with a consequent reduction of oxidative stress. Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Databáze:	MEDLINE
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