Use of hexon as an antigen for the production of monoclonal antibodies capable of detecting multiple adenovirus types.

Autor: Timoshicheva TA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia., Zabrodskaya YA; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia; Petersburg Nuclear Physics Institute named after B. P. Konstantinov of National Research Center 'Kurchatov Institute', 188300, mkr. Orlova roshcha 1, Gatchina, Russia. Electronic address: yana@zabrodskaya.net., Ramsay E; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia., Amosova IV; Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia.
Jazyk: angličtina
Zdroj: Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2019 Mar; Vol. 58, pp. 44-49. Date of Electronic Publication: 2019 Jan 17.
DOI: 10.1016/j.biologicals.2019.01.006
Abstrakt: In this work, monoclonal antibodies to the adenovirus protein hexon were produced, and their biological and diagnostic properties were characterized. The specific activities of the new monoclonal antibodies, with respect to various adenovirus types, were studied by enzyme-linked immunosorbent assay, indirect immunofluorescence, and western blot analysis. The data demonstrate the potential of the monoclonal antibodies developed, namely 4B7 and 6B12, for use in the development of modern diagnostic assays.
(Copyright © 2019 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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