| Autor: |
Herrera KMS; a Laboratório de Microbiologia Médica, Universidade Federal de São João del-Rei (UFSJ) , Campus Centro Oeste Dona Lindu , Divinópolis , Minas Gerais , Brazil., Silva FKD; a Laboratório de Microbiologia Médica, Universidade Federal de São João del-Rei (UFSJ) , Campus Centro Oeste Dona Lindu , Divinópolis , Minas Gerais , Brazil., Oliveira ME; a Laboratório de Microbiologia Médica, Universidade Federal de São João del-Rei (UFSJ) , Campus Centro Oeste Dona Lindu , Divinópolis , Minas Gerais , Brazil., Paiva MC; b Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Universidade Federal de São João del-Rei (UFSJ) , Campus Centro Oeste Dona Lindu , Divinópolis , Minas Gerais , Brazil., Soares AC; c Laboratório de Farmacologia, Universidade Federal de São João del-Rei (UFSJ) , Campus Centro Oeste Dona Lindu , Divinópolis , Minas Gerais , Brazil., Siqueira Ferreira JM; a Laboratório de Microbiologia Médica, Universidade Federal de São João del-Rei (UFSJ) , Campus Centro Oeste Dona Lindu , Divinópolis , Minas Gerais , Brazil. |
| Abstrakt: |
Considering the clinical importance of biofilm in medical devices and chronic infections, this study aimed to investigate the action of polymyxin B on Klebsiella pneumoniae (K. pneumoniae) biofilm. The experiments were performed using a biofilm formation assay and the interaction of polysorbate 80 was explored. Both inhibition of biofilm formation and reduction of pre-formed biofilm occurred in a concentration-dependent manner with inhibition as high as 56 and 64%, and reduction of pre-formed biofilm as high as 70 and 66%, with and without polysorbate, respectively. The addition of polysorbate enhances the biofilm reduction, but more studies are needed to elucidate this mechanism. Our findings reveal, for the first time, polymyxin B as a potential agent for the treatment of K. pneumoniae biofilm, a current challenge for clinical practice. |
