[Efficiency of polyphenol-rich grape products for the prevention of metabolic disorders in the experiment].

Autor:	Kubyshkin AV; Medical Academy of V.I. Vernadsky Crimean Federal University, Simferopol., Avidzba MA; «Magarach» National Research Institute for Grape and Wine, Yalta., Fomochkina II; Medical Academy of V.I. Vernadsky Crimean Federal University, Simferopol., Ogay YA; «Magarach» National Research Institute for Grape and Wine, Yalta., Khanferyan RA; Federal Research Centre of Food and Biotechnology, Moscow., Shramko YI; Medical Academy of V.I. Vernadsky Crimean Federal University, Simferopol., Markosov VA; North Caucasian Regional Research Institute of Horticulture and Viticulture, Krasnodar., Guguchkina TI; North Caucasian Regional Research Institute of Horticulture and Viticulture, Krasnodar., Ageeva NM; North Caucasian Regional Research Institute of Horticulture and Viticulture, Krasnodar., Zaytsev GP; Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow., Chernousova IV; North Caucasian Regional Research Institute of Horticulture and Viticulture, Krasnodar.
Jazyk:	ruština
Zdroj:	Voprosy pitaniia [Vopr Pitan] 2017; Vol. 86 (1), pp. 100-107. Date of Electronic Publication: 2016 Dec 28.
DOI:	10.24411/0042-8833-2017-00027
Abstrakt:	The article presents the results of study of the effect of polyphenol-rich materials obtained from grapes on the clinical symptoms, activity of non-specific proteases, and parameters of lipid peroxidation (LPO) of blood of rats with fructose model of metabolic syndrome (MS). White male rats (n=54, with initial body weight of 190-210 g) were randomly divided into five groups: control and 4 experimental groups. Body weight, circumference of the abdomen, blood serum level of glucose, total cholesterol, triglycerides and high density (HDL) lipoproteins were monitored in all animals in dynamics. Also non-specific proteases and their inhibitors were evaluated in rat blood by enzymatic methods, lipid peroxidation profile (malondialdehyde and caeruloplasmin blood serum level, superoxide dismutase blood activity) - by spectrophotometric methods. Modeling of the MS (10% fructose in drinking water) within 8 weeks resulted in statistically significant increase in body mass, abdominal adipose tissue, the activity of elastase-like (ELA) and trypsin-like (TLA) proteinases (20 and 18% respectively), the level of secondary products of LPO (50%), and decrease in activity of superoxide dismutase (15%) compared with the parameters of intact animals. The use of polyphenol-rich materials obtained from grapes with a total content of 1; 1,1 and 4 mg of phenolic compounds (in 0.05 ml) at MS for 4 weeks contributed regression the key clinical signs of MS: significant decrease of glucose and triglyceride levels against the background of increasing the HDL cholesterol were observed. The polyphenol-rich materials obtained from grapes influenced positively on the proteolytic profile (decreased TLA and ELA by 20% at the maximum dosage), contributed to the increase of the level of acid-stable protease inhibitors (by 21%), and reduced the amount of secondary products of LPO (by 34%). The dose-dependent effect of the amount of polyphenol components contained in food concentrates has been revealed. (Copyright© GEOTAR-Media Publishing Group.)
Databáze:	MEDLINE
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