Biotransformation of 4-nitrophenol by co-immobilized Geobacter sulfurreducens and anthraquinone-2-sulfonate in barium alginate beads.

Autor: Rodriguez SY; Universidad Autonoma de Nuevo Leon (UANL), Facultad de Ciencias Quimicas, Av. Universidad S/N, Cd. Universitaria, San Nicolas de los Garza, 66455, Nuevo Leon, Mexico., Cantú ME; Universidad Autonoma de Nuevo Leon (UANL), Facultad de Ciencias Quimicas, Av. Universidad S/N, Cd. Universitaria, San Nicolas de los Garza, 66455, Nuevo Leon, Mexico., Garcia-Reyes B; Universidad Autonoma de Nuevo Leon (UANL), Facultad de Ciencias Quimicas, Av. Universidad S/N, Cd. Universitaria, San Nicolas de los Garza, 66455, Nuevo Leon, Mexico., Garza-Gonzalez MT; Universidad Autonoma de Nuevo Leon (UANL), Facultad de Ciencias Quimicas, Av. Universidad S/N, Cd. Universitaria, San Nicolas de los Garza, 66455, Nuevo Leon, Mexico., Meza-Escalante ER; Instituto Tecnologico de Sonora (ITSON), Departamento de Ciencias del Agua y Medio Ambiente, 5 de Febrero 818 Sur, C.P. 85000, Cuidad Obregon, Sonora, Mexico., Serrano D; Instituto Tecnologico de Sonora (ITSON), Departamento de Ciencias del Agua y Medio Ambiente, 5 de Febrero 818 Sur, C.P. 85000, Cuidad Obregon, Sonora, Mexico., Alvarez LH; Universidad Autonoma de Nuevo Leon (UANL), Facultad de Ciencias Quimicas, Av. Universidad S/N, Cd. Universitaria, San Nicolas de los Garza, 66455, Nuevo Leon, Mexico; Instituto Tecnologico de Sonora (ITSON), Departamento de Ciencias Agronomicas y Veterinarias, 5 de Febrero 818 Sur, C.P. 85000, Cuidad Obregon, Sonora, Mexico. Electronic address: luis.alvarez@itson.edu.mx.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2019 Apr; Vol. 221, pp. 219-225. Date of Electronic Publication: 2019 Jan 07.
DOI: 10.1016/j.chemosphere.2019.01.041
Abstrakt: Geobacter sulfurreducens and anthraquinone-2-sulfonate (AQS) were used suspended and immobilized in barium alginate during the biotransformation of 4-nitrophenol (4-NP). The assays were conducted at different concentrations of 4-NP (50-400 mg/L) and AQS, either in suspended (0-400 μM) or immobilized form (0 or 760 μM), and under different pH values (5-9). G. sulfurreducens showed low capacity to reduce 4-NP in absence of AQS, especially at the highest concentrations of the contaminant. AQS improved the reduction rates from 0.0086 h -1 , without AQS, to 0.149 h -1  at 400 μM AQS, which represent an increment of 17.3-fold. The co-immobilization of AQS and G. sulfurreducens in barium alginate beads (AQS i -G i ) increased the reduction rates up to 4.8- and 7.2-fold, compared to incubations with G. sulfurreducens in suspended and immobilized form, but in absence of AQS. AQS i -G i provides to G. sulfurreducens a barrier against the possibly inhibiting effects of 4-NP.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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