Glucose-holmium for radiotherapy: Characterization and in vitro assays.

Autor: Dalmazio I; Centro de Desenvolvimento da Tecnologia Nuclear - CDTN. Campus da UFMG, Av. Pres. Antônio Carlos, 6627 - Pampulha, Belo Horizonte, MG 31270-901, Brazil. Electronic address: dalmazio@cdtn.br., Campos TPR; Universidade Federal de Minas Gerais, Departamento de Engenharia Nuclear, Escola de Engenharia, Universidade Federal de Minas Gerais - UFMG, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil. Electronic address: tprcampos@pq.cnpq.br.
Jazyk: angličtina
Zdroj: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine [Appl Radiat Isot] 2019 Mar; Vol. 145, pp. 193-197. Date of Electronic Publication: 2018 Dec 21.
DOI: 10.1016/j.apradiso.2018.12.015
Abstrakt: Background: The existence of saccharide-holmium complexes, containing mono or polysaccharide molecules, is an attractive hypothesis toward a radiation therapy (RT) with beta-emitters targeting high glucose metabolic human sites. To exam such hypothesis, the aim of this study was to investigate the possible chemical interactions of Ho and glucose molecules and if glucose may be a facilitator to holmium cell internalization based on in vitro uptake assays and mass spectrometry analyses.
Methods: The ionic-solution preparations were based on glucose-anhydrous and holmium-nitrate hydrated in aqueous solution, in non-radioactive condition. The uptakes in MDAMB231 cell lineage were evaluated, at 0 and 50 µg mL -1 holmium solution, in incubation times of 10, 30 and 50 min. The measurements of the holmium mass into the dried cell were evaluated by Neutron Activation Analysis - NAA method. Also, the ionic solution was tested in Electrospray Ionization Mass Spectrometry (ESI-MS) in order to identify Ho and glucose interactions.
Results: There were intracellular holmium-uptake in MDAMB-231 of 3.6 ± 0.1, 6.8 ± 0.2 and 9.7 ± 0.3 μg increasing linearly with incubation time. The m/z ions at 523, 586, 649, 991 and 1054 were attributed to the positively loaded species containing Ho +3 , glucose (GLU) and NO 3 - , making up the possible molecular compound formulae, involving Ho, GLU, and anions.
Conclusions: The findings of the in vitro assay and the ESI-MS suggested a suitable holmium cell uptake, increased in function of incubation time, due to the presence of glucose and holmium chemical interactions in solution.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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