An In Vitro Versus In Vivo Toxicogenomic Investigation of Prenatal Exposures to Tobacco Smoke.
| Autor: | Perry IA; School of Biosciences, Cardiff University, Museum Avenue, Cardiff, United Kingdom., Sexton KJ; School of Biosciences, Cardiff University, Museum Avenue, Cardiff, United Kingdom., Prytherch ZC; School of Biosciences, Cardiff University, Museum Avenue, Cardiff, United Kingdom., Blum JL; Department of Environmental Medicine, NYU School of Medicine, NYU Langone Medical Centre, Tuxedo, New York., Zelikoff JT; Department of Environmental Medicine, NYU School of Medicine, NYU Langone Medical Centre, Tuxedo, New York., BéruBé KA; School of Biosciences, Cardiff University, Museum Avenue, Cardiff, United Kingdom. |
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| Jazyk: | angličtina |
| Zdroj: | Applied in vitro toxicology [Appl In Vitro Toxicol] 2018 Dec; Vol. 4 (4), pp. 379-388. Date of Electronic Publication: 2017 May 18. |
| DOI: | 10.1089/aivt.2016.0041 |
| Abstrakt: | Approximately 1 million women smoke during pregnancy despite evidence demonstrating serious juvenile and/or adult diseases being linked to early-life exposure to cigarette smoke. Susceptibility could be determined by factors in previous generations, that is, prenatal or "maternal" exposures to toxins. Prenatal exposure to airborne pollutants such as mainstream cigarette smoke has been shown to induce early-life insults (i.e., gene changes) in Offspring that serve as biomarkers for disease later in life. In this investigation, we have evaluated genome-wide changes in the lungs of mouse Dams and their juvenile Offspring exposed prenatally to mainstream cigarette smoke. An additional lung model was tested alongside the murine model, as a means to find an alternative in vitro , human tissue-based replacement for the use of animals in medical research. Our toxicogenomic and bio-informatic results indicated that in utero exposure altered the genetic patterns of the fetus, which could put them at greater risk for developing a range of chronic illnesses in later life. The genes altered in the in vitro , cell culture model were reflected in the murine model of prenatal exposure to mainstream cigarette smoke. The use of alternative in vitro models derived from human medical waste tissues could be viable options to achieve human endpoint data and conduct research that meets the remits for scientists to undertake the 3Rs practices. Competing Interests: Author Disclosure Statement No competing financial interests exist. |
| Databáze: | MEDLINE |
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