Mucin-coating technologies for protection and reduced aggregation of cellular production systems.

Autor: Shurer CR; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York., Head SE; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York., Goudge MC; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York., Paszek MJ; Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York.; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York.; Field of Biophysics, Cornell University, Ithaca, New York.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2019 May; Vol. 116 (5), pp. 994-1005. Date of Electronic Publication: 2019 Feb 19.
DOI: 10.1002/bit.26916
Abstrakt: Optimization of host-cell production systems with improved yield and production reliability is desired to meet the increasing demand for biologics with complex posttranslational modifications. Aggregation of suspension-adapted mammalian cells remains a significant problem that can limit the cellular density and per volume yield of bioreactors. Here, we propose a genetically encoded technology that directs the synthesis of antiadhesive and protective coatings on the cellular surface. Inspired by the natural ability of mucin glycoproteins to resist cellular adhesion and hydrate and protect cell and tissue surfaces, we genetically encode new cell-surface coatings through the fusion of engineered mucin domains to synthetic transmembrane anchors. Combined with appropriate expression systems, the mucin-coating technology directs the assembly of thick, highly hydrated barriers to strongly mitigate cell aggregation and protect cells in suspension against fluid shear stresses. The coating technology is demonstrated on suspension-adapted human 293-F cells, which resist clumping even in media formulations that otherwise would induce extreme cell aggregation and show improved performance over a commercially available anticlumping agent. The stable biopolymer coatings do not show deleterious effects on cell proliferation rate, efficiency of transient transfection with complementary DNAs, or recombinant protein expression. Overall, our mucin-coating technology and engineered cell lines have the potential to improve the single-cell growth and viability of suspended cells in bioreactors.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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