| Autor: |
Tang S; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China. srtang@fjmu.edu.cn., You X; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China. hhyou4906@163.com., Fang Q; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China. 18250485385@163.com., Li X; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China. lx281032@163.com., Li G; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China. lgw6301@fjmu.edu.cn., Chen J; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China. cjh_huaxue@126.com., Chen W; Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China. chenandhu@163.com. |
| Abstrakt: |
A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS₂ QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO₂ NSs) were prepared by exfoliating the bulk δ-MnO₂ material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS₂ QDs could be quenched in the presence of MnO₂ NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO₂ NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20⁻2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis. |
