Mycobacterium leprae Recombinant Antigen Induces High Expression of Multifunction T Lymphocytes and Is Promising as a Specific Vaccine for Leprosy.

Autor:	Bezerra-Santos M; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil.; Department of Health Education, Federal University of Sergipe, Lagarto, Brazil., do Vale-Simon M; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil., Barreto AS; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil., Cazzaniga RA; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil., de Oliveira DT; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil., Barrios MR; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil., Ferreira AR; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil.; Infectious Disease Research Institute, Seattle, WA, United States., Santos-Bio NC; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil.; Infectious Disease Research Institute, Seattle, WA, United States., Reed SG; Instituto de Investigação em Imunologia, INCT-CNPq, São Paulo, Brazil., de Almeida RP; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil.; Infectious Disease Research Institute, Seattle, WA, United States.; Department of Medicine, Federal University of Sergipe, Aracaju, Brazil., Corrêa CB; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil., Duthie MS; Instituto de Investigação em Imunologia, INCT-CNPq, São Paulo, Brazil., de Jesus AR; Laboratory of Immunology and Molecular Biology, Federal University of Sergipe, Aracaju, Brazil.; Infectious Disease Research Institute, Seattle, WA, United States.; Department of Medicine, Federal University of Sergipe, Aracaju, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2018 Dec 12; Vol. 9, pp. 2920. Date of Electronic Publication: 2018 Dec 12 (Print Publication: 2018).
DOI:	10.3389/fimmu.2018.02920
Abstrakt:	Leprosy is a chronic disease caused by M. leprae infection that can cause severe neurological complications and physical disabilities. A leprosy-specific vaccine would be an important component within control programs but is still lacking. Given that multifunctional CD4 T cells [i.e., those capable of simultaneously secreting combinations of interferon (IFN)-γ, interleukin (IL)-2, and tumor necrosis factor (TNF)] have now been implicated in the protective response to several infections, we tested the hypothesis if a recombinant M. leprae antigen-specific multifunctional T cells differed between leprosy patients and their healthy contacts. We used whole blood assays and peripheral blood mononuclear cells to characterize the antigen-specific T cell responses of 39 paucibacillary (PB) and 17 multibacillary (MB) leprosy patients and 31 healthy household contacts (HHC). Cells were incubated with either crude mycobacterial extracts ( M. leprae cell sonicate-MLCS) and purified protein derivative (PPD) or recombinant ML2028 protein, the homolog of M. tuberculosis Ag85B. Multiplex assay revealed antigen-specific production of IFN-γ and IL-2 from cells of HHC and PB, confirming a Th1 bias within these individuals. Multiparameter flow cytometry then revealed that the population of multifunctional ML2028-specific T cells observed in HHC was larger than that observed in PB patients. Taken together, our data suggest that these multifunctional antigen-specific T cells provide a more effective response against M. leprae infection that prevents the development of leprosy. These data further our understanding of M. leprae infection/leprosy and are instructive for vaccine development.
Databáze:	MEDLINE
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