| Autor: |
Morelli L; Department of Micro- and Nanotechnology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. lidiamorelli90@gmail.com., Centorbi FA, Ilchenko O, Jendresen CB, Demarchi D, Nielsen AT, Zór K, Boisen A |
| Jazyk: |
angličtina |
| Zdroj: |
The Analyst [Analyst] 2019 Feb 25; Vol. 144 (5), pp. 1600-1607. |
| DOI: |
10.1039/c8an02128g |
| Abstrakt: |
Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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