| Autor: |
Tatenaka Y; Dojindo Laboratories , Tabaru 2025-5 , Mashiki-machi , Kumamoto 861-2202 , Japan., Kato H; Laboratory of Biochemistry and Molecular Biology, Department of Medical Sciences , University of Miyazaki , 5200 Kihara Kiyotake , Miyazaki 889-1692 , Japan., Ishiyama M; Dojindo Laboratories , Tabaru 2025-5 , Mashiki-machi , Kumamoto 861-2202 , Japan., Sasamoto K; Dojindo Laboratories , Tabaru 2025-5 , Mashiki-machi , Kumamoto 861-2202 , Japan., Shiga M; Dojindo Laboratories , Tabaru 2025-5 , Mashiki-machi , Kumamoto 861-2202 , Japan., Nishitoh H; Laboratory of Biochemistry and Molecular Biology, Department of Medical Sciences , University of Miyazaki , 5200 Kihara Kiyotake , Miyazaki 889-1692 , Japan., Ueno Y; Dojindo Laboratories , Tabaru 2025-5 , Mashiki-machi , Kumamoto 861-2202 , Japan. |
| Abstrakt: |
We have developed three types of lipid droplet (LD)-specific fluorescent probes for live-cell imaging, Lipi-Blue, Lipi-Green, and Lipi-Red, which exhibit fluorescence upon being incorporated into LDs both of living and of fixed cells. These Lipi-probes are LD-specific probes that contain a pyrene or perylene group as a fluorescent scaffold and can be used to observe dynamics of LD in live cells and also interrelations with other organelles by simultaneous staining with multiple organelle-specific probes. Additionally, Lipi-Blue and Lipi-Green allow monitoring LDs in live cells even for 48 h after the staining. Here we show that newly formed LDs and previously existed LDs can be separately monitored in a single cell by using these probes and that intercellular transfer of whole LDs is observed in KB cells, but not in HepG2 cells under the same culturing condition. These findings indicate that newly developed LD-specific probes are useful to analyze the dynamics of LDs in live cells. |
