| Autor: |
da Silva MV; Department of Parasitology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, Brazil., de Almeida VL; Institute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, Brazil., de Oliveira WD; Institute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, Brazil., Matos Cascudo NC; Institute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, Brazil., de Oliveira PG; Institute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, Brazil., da Silva CA; General Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, Brazil., da Silva ACS; General Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, Brazil., Dos Reis Monteiro MLG; General Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, Brazil., Correa RRM; General Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, Brazil., Oliveira MAP; Institute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, Brazil., Lino-Júnior RS; Institute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, Brazil., Celes MRN; Institute of Tropical Pathology and Public Health of Federal University of Goiás, Federal University of Goiás, 74605-050 Goiânia, Brazil., Machado JR; General Pathology, Institute of Natural and Biological Sciences, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, Brazil. |
| Abstrakt: |
Inflammatory response in Chagas disease is related to parasite and host factors. However, immune system regulation has not been fully elucidated. Thus, this study is aimed at evaluating IL-4 influence on acute phase of Trypanosoma cruzi experimental infection through dosage of cytokine levels in cardiac homogenate of infected Balb/c WT and Balb/c IL-4 -/- as well as its histopathological repercussions. For such purpose, mice were divided into two groups: an infected group with 100 forms of the Colombian strain and an uninfected group. After 21 days of infection, animals were euthanized and the blood, spleen, and heart were collected. The spleen was used to culture splenic cells in 48 h. Subsequently, cytokines TNF- α , IL-12p70, IL-10, IFN- γ , and IL-17 were measured in the blood, culture supernatant, and heart apex by ELISA. The base of the heart was used for histopathological analysis. From these analysis, infected Balb/c IL-4 -/- mice showed milder inflammatory infiltrate compared to Balb/c WT, but without changes in nest density and collagen deposition. IL-4 absence culminated in lower cardiac tissue IFN- γ production, although it did not affect TNF- α expression in situ. It also decreased TNF- α systemic production and increased IL-10, both systemically and in situ . In addition, IL-4 absence did not influence IL-17 expression. Splenocytes of IL-4-deficient mice produced higher amounts of IFN- γ , TNF- α , and IL-17 and lower amounts of IL-10. Thus, IL-4 absence in acute phase of experimental infection with T. cruzi Colombian strain reduces myocarditis due to lower IFN- γ production and greater IL-10 production in situ and this pattern is not influenced by splenocyte general repertoire. |
