Xylitol Production from Lignocellulosic Pentosans: A Rational Strain Engineering Approach toward a Multiproduct Biorefinery.

Autor:	Dasgupta D; Biotechnology Conversion Area, Bio Fuels Division , CSIR-Indian Institute of Petroleum , Dehradun , Uttarakhand 248005 , India., Junghare V, Nautiyal AK; Biotechnology Conversion Area, Bio Fuels Division , CSIR-Indian Institute of Petroleum , Dehradun , Uttarakhand 248005 , India., Jana A; Biotechnology Conversion Area, Bio Fuels Division , CSIR-Indian Institute of Petroleum , Dehradun , Uttarakhand 248005 , India., Hazra S, Ghosh D; Biotechnology Conversion Area, Bio Fuels Division , CSIR-Indian Institute of Petroleum , Dehradun , Uttarakhand 248005 , India.
Jazyk:	angličtina
Zdroj:	Journal of agricultural and food chemistry [J Agric Food Chem] 2019 Jan 30; Vol. 67 (4), pp. 1173-1186. Date of Electronic Publication: 2019 Jan 16.
DOI:	10.1021/acs.jafc.8b05509
Abstrakt:	Kluyveromyces marxianus IIPE453 can utilize biomass-derived fermentable sugars for xylitol and ethanol fermentation. In this study, the xylitol production in the native strain was improved by overexpression of endogenous d-xylose reductase gene. A suitable expression cassette harboring the gene of interest was constructed and incorporated in the native yeast. qPCR analysis demonstrated the 2.1-fold enhancement in d-xylose reductase transcript levels in the modified strain with 1.62-fold enhancement in overall xylitol yield without affecting its ethanol fermenting capacity. Material balance analysis on 2 kg of sugar cane bagasse-derived fermentable sugars illustrated an excess of 58.62 ± 0.15 g of xylitol production by transformed strain in comparison to the wild variety with similar ethanol yield. The modified strain can be suitably used as a single biocatalyst for multiproduct biorefinery application.
Databáze:	MEDLINE
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