Heterozygosity testing and multiplex DNA panel screening as a potential tool to monitor health and inbreeding in a small, closed dog population.

Autor: Keijser SFA; 1Expertise Centre Genetics of Companion Animals, Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM Utrecht, The Netherlands., Fieten H; 1Expertise Centre Genetics of Companion Animals, Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM Utrecht, The Netherlands., Vos-Loohuis M; 2Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM Utrecht, The Netherlands., Piek CJ; 2Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM Utrecht, The Netherlands., Anderson H; Genoscoper Laboratories Oy, P.O. Box 1040, 00251 Helsinki, Finland., Donner J; Genoscoper Laboratories Oy, P.O. Box 1040, 00251 Helsinki, Finland., Scholten I; Dutch Shepherd Dog Club, Vijfsprongweg 126, 6741 JC Lunteren, The Netherlands., Nielen M; 5Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 107, 3584 CM Utrecht, The Netherlands., Hesselink JW; 2Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM Utrecht, The Netherlands., van Steenbeek FG; 2Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 108, 3584 CM Utrecht, The Netherlands.
Jazyk: angličtina
Zdroj: Canine genetics and epidemiology [Canine Genet Epidemiol] 2018 Dec 28; Vol. 5, pp. 12. Date of Electronic Publication: 2018 Dec 28 (Print Publication: 2018).
DOI: 10.1186/s40575-018-0068-6
Abstrakt: Background: Selective breeding in populations with a limited effective population size may result in a loss of genetic diversity, which can cause an increased concentration of specific disease liability genes. The Dutch Shepherd Dog (DSD) in the Netherlands is an example of such a breed with a small effective population.
Objective: To evaluate the measurement of genetic diversity and multiplex DNA panel screening for implementation in a breeding strategy for the Dutch Shepherd Dog (DSD) and to investigate the clinical relevance of potentially identified mutations in the multiplex DNA panel screening.
Results: Genome-wide SNP testing showed genetic isolation and reduced genetic diversity within coat variety subgroups of the DSD. Panel screening identified a Von Willebrand's Disease type I mutation. Although decreased Von Willebrand's Factor proteins were significantly lower in DSDs carrying the VWD-I allele compared to the wildtype, clinical follow-up did not show a significant association between the clinical phenotype and VWD-I genotype.
Conclusions: Genetic relationship measurement within a breed population may be a useful tool to enable breeding strategies to conserve genetic diversity. Results from a disease panel screening need to be evaluated for clinical relevance before breed selection restrictions can be considered.
Competing Interests: Samples were collected with the informed consent of the owners.Not applicable.HA and JD are employees of Genoscoper Laboratories Oy, developer of the MyDogDNA™ screening service. “The aforementioned authors played no role in the decision to conduct the study or use the MyDogDNA™ genotyping platform, the original study design, or writing of the original manuscript draft.”Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Databáze: MEDLINE
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