Ramification of zinc oxide doped hydroxyapatite biocomposites for the mineralization of osteoblasts.

Autor: Gnaneshwar PV; Centre for Nanofibers & Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore, Singapore; Department of Physics and Nanotechnology, SRM University, Kattankulathur, Chennai, India; Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica and National Tsing Hua University, Taiwan., Sudakaran SV; Centre for Nanofibers & Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore, Singapore., Abisegapriyan S; Centre for Nanofibers & Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore, Singapore., Sherine J; Department of Physics and Nanotechnology, SRM University, Kattankulathur, Chennai, India., Ramakrishna S; Centre for Nanofibers & Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore, Singapore., Rahim MHA; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300, Gambang, Kuantan, Pahang, Malaysia., Yusoff MM; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300, Gambang, Kuantan, Pahang, Malaysia., Jose R; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300, Gambang, Kuantan, Pahang, Malaysia., Venugopal JR; Centre for Nanofibers & Nanotechnology, Mechanical Engineering, Faculty of Engineering, National University of Singapore, Singapore; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300, Gambang, Kuantan, Pahang, Malaysia. Electronic address: venugopal@ump.edu.my.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2019 Mar; Vol. 96, pp. 337-346. Date of Electronic Publication: 2018 Nov 24.
DOI: 10.1016/j.msec.2018.11.033
Abstrakt: Far-flung evolution in tissue engineering enabled the development of bioactive and biodegradable materials to generate biocomposite nanofibrous scaffolds for bone repair and replacement therapies. Polymeric bioactive nanofibers are to biomimic the native extracellular matrix (ECM), delivering tremendous regenerative potentials for drug delivery and tissue engineering applications. It's been known from few decades that Zinc oxide (ZnO) nanoparticles are enhancing bone growth and providing proliferation of osteoblasts when incorporated with hydroxyapatite (HAp). We attempted to investigate the interaction between the human foetal osteoblasts (hFOB) with ZnO doped HAp incorporated biocomposite poly(L-lactic acid)-co-poly(ε-caprolactone) and silk fibroin (PLACL/SF) nanofibrous scaffolds for osteoblasts mineralization in bone tissue regeneration. The present study, we doped ZnO with HAp (ZnO(HAp) using the sol-gel ethanol condensation technique. The properties of PLACL/SF/ZnO(HAp) biocomposite nanofibrous scaffolds enhanced with doped and blended ZnO/HAp were characterized using Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Contact angle and Tensile studies to determine the morphology, functionality, wettability and stability. The in vitro study results showed that the addition of ZnO and HAp enhances the secretion of bone mineral matrix (98%) with smaller fiber diameter (139.4 ± 27 nm) due to the presence of silk fibroin showing potential tensile properties (322.4%), and increased the proliferation of osteoblasts for bone tissue regeneration.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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