New compounds of Siolmatra brasiliensis and inhibition of in vitro protein glycation damage.

Autor: Dos Santos CHC; Programa de Pós-graduação em Química, Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica 23897-000, RJ, Brazil. Electronic address: carloshcs@ufrrj.br., Talpo TC; Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, UNESP, Araraquara 14800-903, SP, Brazil., Motta BP; Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, UNESP, Araraquara 14800-903, SP, Brazil., Kaga AK; Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, UNESP, Araraquara 14800-903, SP, Brazil., Baviera AM; Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, UNESP, Araraquara 14800-903, SP, Brazil. Electronic address: amanda.baviera@unesp.br., Castro RN; Programa de Pós-graduação em Química, Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica 23897-000, RJ, Brazil., da Silva VC; Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá 78060-900, MT, Brazil., de Sousa-Junior PT; Departamento de Química, Universidade Federal de Mato Grosso, Cuiabá 78060-900, MT, Brazil., Wessjohann L; Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle (Saale) D-06120, Germany., de Carvalho MG; Programa de Pós-graduação em Química, Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica 23897-000, RJ, Brazil.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2019 Mar; Vol. 133, pp. 109-119. Date of Electronic Publication: 2018 Dec 31.
DOI: 10.1016/j.fitote.2018.12.023
Abstrakt: Twenty compounds were isolated from the hydroethanolic extract of the stems of Siolmatra brasiliensis, five flavonoids, two lignans, one glucosyl phytosterol, seven nor-cucurbitacins, one new phenolic derivative named siolmatrin (1) and four new dammarane-type saponins named siolmatrosides II-V (2-5), the structures of the compounds were assigned by means of 1D and 2D NMR experiments and HRESIMS of the natural compounds and some acetyl derivatives. The effects of the crude hydroethanolic extract (SbExt) and the ethyl acetate fraction (SbEtAc) of Siolmatra brasiliensis stems on the formation of advanced glycation end-products (AGEs) were also investigated. In the in vitro model system of protein glycation using bovine serum albumin (BSA) and glucose, addition of SbExt or SbEtAc inhibited the formation of fluorescent AGEs, in parallel to minor levels of fructosamine (SbEtAc) and markers of tyrosine and tryptophan oxidation (SbExt and SbEtAc). Protein crosslinking, which represents changes of late stages of protein glycation, was reduced in the presence of SbExt and SbEtAc. Siolmatra brasiliensis stems seem to be a promising source of compounds having ability to prevent glycoxidation changes, arising as an interesting option to be studied as a complementary therapy for complications of diabetes.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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