Glucosamine Hydrochloride and N -Acetylglucosamine Influence the Response of Bovine Chondrocytes to TGF-β3 and IGF in Monolayer and Three-Dimensional Tissue Culture.

Autor: Pizzolatti ALA; 1Laboratory of Biomechanical Engineering (LEBm), University Hospital, Department of Mechanical Engineering, Federal University of Santa Catarina, St. Maria Flora Pausewang, Florianópolis, SC 88036-800 Brazil.; 2CAPES Foundation, Ministry of Education of Brazil, St. ERL-Norte, Brasília, DF 70.040-020 Brazil., Gaudig F; Friedrich Baur Biomed Center, Bayreuth, St. Ludwig-Thoma- 36c, 95447 Bayreuth, Bavaria Germany.; 4University of Bayreuth, St. University 30, 95447 Bayreuth, Bavaria Germany., Seitz D; Friedrich Baur Biomed Center, Bayreuth, St. Ludwig-Thoma- 36c, 95447 Bayreuth, Bavaria Germany.; 4University of Bayreuth, St. University 30, 95447 Bayreuth, Bavaria Germany., Roesler CRM; 1Laboratory of Biomechanical Engineering (LEBm), University Hospital, Department of Mechanical Engineering, Federal University of Santa Catarina, St. Maria Flora Pausewang, Florianópolis, SC 88036-800 Brazil., Salmoria GV; 1Laboratory of Biomechanical Engineering (LEBm), University Hospital, Department of Mechanical Engineering, Federal University of Santa Catarina, St. Maria Flora Pausewang, Florianópolis, SC 88036-800 Brazil.
Jazyk: angličtina
Zdroj: Tissue engineering and regenerative medicine [Tissue Eng Regen Med] 2018 Aug 30; Vol. 15 (6), pp. 781-791. Date of Electronic Publication: 2018 Aug 30 (Print Publication: 2018).
DOI: 10.1007/s13770-018-0150-x
Abstrakt: Background: Glucosamine hydrochloride (GlcN·HCl) has been shown to inhibit cell growth and matrix synthesis, but not with N -acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical growth factors (GF), or to a different metabolization of the two glucosamine derivatives. The aim of the present study was to evaluate the synergy between GlcN·HCl, GlcNAc, and GF on proliferation and cartilage matrix synthesis.
Method: Bovine chondrocytes were cultivated in monolayers for 48 h and in three-dimensional (3D) chitosan scaffolds for 30 days in perfusion bioreactors. Serum-free (SF) medium was supplemented with either growth factors (GF) TGF-β (5 ng mL -1 ) and IGF-I (10 ng mL -1 ), GlcN·HCl or GlcNAc at 1mM each or both. Six groups were compared according to medium supplementation: (a) SF control; (b) SF + GlcN·HCl; (c) SF + GlcNAc; (d) SF + GF; (e) SF + GF + GlcN·HCl; and (f) SF + GF + GlcNAc. Cell proliferation, proteoglycan, collagen I (COL1), and collagen II (COL2) synthesis were evaluated.
Results: The two glucosamines showed opposite effects in monolayer culture: GlcN·HCl significantly reduced proliferation and GlcNAc significantly augmented cellular metabolism. In the 30 days 3D culture, the GlcN·HCl added to GF stimulated cell proliferation more than when compared to GF only, but the proteoglycan synthesis was smaller than GF. However, GlcNAc added to GF improved the cell proliferation and proteoglycan synthesis more than when compared to GF and GF/GlcN·HCl. The synthesis of COL1 and COL2 was observed in all groups containing GF.
Conclusion: GlcN·HCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis.
Competing Interests: The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.There are no animal experiments carried out for this article.
Databáze: MEDLINE
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