IgG1 and IgG4 antibodies against Aedes aegypti salivary proteins and risk for dengue infections.

Autor: Cardenas JC; Laboratorio Clínico, Hospital Local Los Patios, Norte de Santander, Colombia.; Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America., Drame PM; Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America., Luque-Burgos KA; Laboratorio Clínico, Hospital Erasmo Meoz de Cúcuta, Norte de Santander, Colombia., Berrio JD; Laboratorio Clínico, Hospital Erasmo Meoz de Cúcuta, Norte de Santander, Colombia., Entrena-Mutis E; Laboratorio Clínico, Hospital Erasmo Meoz de Cúcuta, Norte de Santander, Colombia., González MU; Laboratorio Clínico, Hospital Erasmo Meoz de Cúcuta, Norte de Santander, Colombia., Carvajal DJ; Grupo Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Pamplona, Norte de Santander, Colombia., Gutiérrez-Silva LY; Laboratorio Clinico, E.S.E Hospital Emiro Quintero Cañizares, Ocaña, Norte de Santander, Colombia., Cardenas LD; Grupo Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Pamplona, Norte de Santander, Colombia., Colpitts TM; National Emerging Infectious Diseases Laboratories (NEIDL), Department of Microbiology, Boston University School of Medicine, Boston, MA, United States of America., Mores CN; Department of Global Health, Milken Institute School of Public Health, The George Washington University, Washington DC, United States of America., Londono-Renteria B; Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Jan 02; Vol. 14 (1), pp. e0208455. Date of Electronic Publication: 2019 Jan 02 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0208455
Abstrakt: Dengue virus (DENV) is an arbovirus responsible for a significant number of deaths in Latin America. This virus is transmitted through the bite of Aedes aegypti, the main mosquito vector, and Ae. albopictus. During blood uptake, the mosquito injects its saliva into the host to facilitate the feeding process. Mosquito saliva contains potent immunogens capable of inducing antibody production directly related to mosquito bite exposure intensity and disease risk. In this study, we first determined the DENV infection status by two different DENV non-structural protein 1 (NS1) based rapid tests and qRT-PCR, then measured the levels of IgG1 and IgG4 antibodies against salivary proteins of Ae. aegypti female mosquitoes in volunteers living in a dengue endemic area. Our results show that people with a positive DENV diagnosis present higher levels of IgG4 antibodies than people with a negative diagnostic test, and that these antibody levels were higher in people with secondary DENV infections. With this study, we show that detection of IgG4 antibodies against mosquito saliva may be a reliable method to evaluate the risk of dengue infection.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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