Prospection of Enzyme Modulators in Aqueous and Ethanolic Extracts of Lippia sidoides Leaves: Genotoxicity, Digestion, Inflammation, and Hemostasis.

Autor: Aparecida Braga M; Biochemistry Laboratory, Department of Chemistry, Universidade Federal de Lavras (UFLA), Campus Universitario, CEP: 3037, Lavras, 37200-000, Brazil., Silva de Abreu T; Biochemistry Laboratory, Department of Chemistry, Universidade Federal de Lavras (UFLA), Campus Universitario, CEP: 3037, Lavras, 37200-000, Brazil., Cardoso Trento MV; Biochemistry Laboratory, Department of Chemistry, Universidade Federal de Lavras (UFLA), Campus Universitario, CEP: 3037, Lavras, 37200-000, Brazil., Henrique Andrade Machado G; Biochemistry Laboratory, Department of Chemistry, Universidade Federal de Lavras (UFLA), Campus Universitario, CEP: 3037, Lavras, 37200-000, Brazil., Lopes Silva Pereira L; Biochemistry Laboratory, Department of Chemistry, Universidade Federal de Lavras (UFLA), Campus Universitario, CEP: 3037, Lavras, 37200-000, Brazil., Assaid Simão A; Biochemistry Laboratory, Department of Chemistry, Universidade Federal de Lavras (UFLA), Campus Universitario, CEP: 3037, Lavras, 37200-000, Brazil., Marcussi S; Biochemistry Laboratory, Department of Chemistry, Universidade Federal de Lavras (UFLA), Campus Universitario, CEP: 3037, Lavras, 37200-000, Brazil.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2019 Mar; Vol. 16 (3), pp. e1800558. Date of Electronic Publication: 2019 Feb 20.
DOI: 10.1002/cbdv.201800558
Abstrakt: The aqueous and ethanolic extracts of Lippia sidoides Cham. were chemically characterized and tested for their action on enzymes involved in processes such as inflammation, blood coagulation, and digestion. Both extracts potentiated the activity of phospholipases A 2 present in the venom of Bothrops atrox in 12 % and completely inhibited the hemolysis induced by B. jararacussu and B. moojeni venoms in the proportions between 1 : 0.5 and 1 : 5 (venom/extracts (w/w)). They inhibited the thrombolysis induced by B. moojeni (10 to 25 %), potentiated the thrombolysis induced by the Lachesis muta muta venom (30 to 80 %), prolonged the coagulation time induced by B. moojeni and L. muta muta venoms, and presented antigenotoxic action. Both extracts reduced the activity of α-glycosidases, the aqueous extract inhibited lipases, and the ethanolic extract inhibited α-amylases. The results demonstrate the modulatory action of the extracts on proteases, phospholipases, and digestive enzymes. In addition, the rich phenolic composition of these extracts highlights their potential for nutraceutical use.
(© 2019 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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