Multiple interspecific hybridization and microsatellite mutations provide clonal diversity in the parthenogenetic rock lizard Darevskia armeniaca.

Autor: Girnyk AE; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia., Vergun AA; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.; Department of Biochemistry, Molecular biology and Genetics, Institute of biology and chemistry, Moscow State Pedagogical University, M. Pirogovskaya Str., 1/1, Moscow, 119991, Russia., Semyenova SK; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia., Guliaev AS; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia., Arakelyan MS; Faculty of Biology, Yerevan State University, 1 Alex Manoogian, 0025, Yerevan, Armenia., Danielyan FD; Faculty of Biology, Yerevan State University, 1 Alex Manoogian, 0025, Yerevan, Armenia., Martirosyan IA; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia., Murphy RW; Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON, M5S 2C6, Canada., Ryskov AP; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia. ryskov@mail.ru.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2018 Dec 29; Vol. 19 (1), pp. 979. Date of Electronic Publication: 2018 Dec 29.
DOI: 10.1186/s12864-018-5359-5
Abstrakt: Background: The parthenogenetic Caucasian rock lizard Darevskia armeniaca, like most other parthenogenetic vertebrate species, originated through interspecific hybridization between the closely related sexual Darevskia mixta and Darevskia valentini. Darevskia armeniaca was shown to consist of one widespread allozyme clone and a few rare ones, but notwithstanding the origin of clonal diversity remains unclear. We conduct genomic analysis of D. armeniaca and its parental sexual species using microsatellite and SNP markers to identify the origin of parthenogenetic clonal lineages.
Results: Four microsatellite-containing loci were genotyped for 111 specimens of D. armeniaca, 17 D. valentini, and four D. mixta. For these species, a total of 47 alleles were isolated and sequenced. Analysis of the data revealed 13 genotypes or presumptive clones in parthenogenetic D. armeniaca, including one widespread clone, two apparently geographically restricted clones, and ten rare clones. Comparisons of genotype-specific markers in D. armeniaca with those of its parental species revealed three founder-events including a common and two rare clones. All other clones appeared to have originated via post-formation microsatellite mutations in the course of evolutionary history of D. armeniaca.
Conclusion: Our new approach to microsatellite genotyping reveals allele-specific microsatellite and SNP markers for each locus studied. Interspecies comparison of these markers identifies alleles inherited by parthenospecies from parental species, and provides new information on origin and evolution of clonal diversity in D. armeniaca. SNP analyses reveal at least three interspecific origins of D. armeniaca, and microsatellite mutations in these initial clones give rise to new clones. Thus, we first establish multiple origins of D. armeniaca. Our study identifies the most effective molecular markers for elucidating the origins of clonal diversity in other unisexual species that arose via interspecific hybridization.
Databáze: MEDLINE
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