| Autor: |
Lopes-Rodrigues M; a Laboratoire de Chimie Physique des Biomolécules, Unité de Chimie Physique Théorique et Structurale (UCPTS), University of Namur , Namur , Belgium.; b Namur Institute of Structured Matter, University of Namur , Namur , Belgium.; c Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya , Barcelona , Spain.; d Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya , Barcelona , Spain., Zanuy D; c Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya , Barcelona , Spain., Alemán C; c Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya , Barcelona , Spain.; d Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya , Barcelona , Spain., Michaux C; a Laboratoire de Chimie Physique des Biomolécules, Unité de Chimie Physique Théorique et Structurale (UCPTS), University of Namur , Namur , Belgium.; b Namur Institute of Structured Matter, University of Namur , Namur , Belgium.; e Namur Research Institute for Life Sciences, University of Namur , Namur , Belgium., Perpète EA; a Laboratoire de Chimie Physique des Biomolécules, Unité de Chimie Physique Théorique et Structurale (UCPTS), University of Namur , Namur , Belgium.; b Namur Institute of Structured Matter, University of Namur , Namur , Belgium.; f Institute of Life-Earth-Environment, University of Namur , Namur , Belgium. |
| Abstrakt: |
Brucella melitensis is a pathogenic bacterium responsible for brucellosis in mammals and humans. Its outer membrane proteins (Omp) control the diffusion of solutes through the membrane, and they consequently have a crucial role in the design of diagnostics and vaccines. Moreover, such proteins have recently revealed their potential for protein-based biomaterials. In the present contribution, the structure of the B. melitensis porin Omp2a is built using the RaptorX threading method. This is a 16-stranded β-barrel with an α-helix on the third loop folding inside the barrel and forming the constriction zone of the channel, a typical feature of general porins such as PhoE and OmpF. The preferential diffusion of cations over anions experimentally observed in anterior studies is evidenced by the presence of distinct clusters of charges in the extracellular loops and in the inner pore. Docking studies support the previously reported hypothesis of Omp2a ability to aid maltotetraose diffusion. The monomer model is then assembled into a homotrimer, stabilized by the L2 loop involved in most of the interface interactions. The stability of the trimer is evaluated in three bilayers: pure 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), pure 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and a mixture of 1:1 of POPC/POPE. All-atom molecular dynamics simulations demonstrate the β-barrel-structural stability over time even though a breathing-like motion is observed. Compared to the pure bilayers, the POPC/POPE better preserves the integrity of the protein and its channel. Overall, this work demonstrates the relevancy of the Omp2a model and will help to design new therapeutic agents and bioinspired nanomaterials. |
