| Autor: |
Dahham SS; EMAN Testing and Research Laboratories, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Malaysia., Al-Rawi SS; EMAN Testing and Research Laboratories, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Malaysia.; Biology Department, University of Zakho, Duhok, Kurdistan Region, Iraq., Ibrahim AH; EMAN Testing and Research Laboratories, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Malaysia.; Integrative Medicine, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Malaysia.; Biology Department, University of Zakho, Duhok, Kurdistan Region, Iraq., Abdul Majid AS; Integrative Medicine, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Malaysia., Abdul Majid AMS; EMAN Testing and Research Laboratories, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Malaysia. |
| Jazyk: |
angličtina |
| Zdroj: |
Saudi journal of biological sciences [Saudi J Biol Sci] 2018 Dec; Vol. 25 (8), pp. 1524-1534. Date of Electronic Publication: 2016 Jan 22. |
| DOI: |
10.1016/j.sjbs.2016.01.031 |
| Abstrakt: |
Desert truffles are seasonal and important edible fungi that grow wild in many countries around the world. Truffles are natural food sources that have significant compositions. In this work, the antioxidant, chemical composition, anticancer, and antiangiogenesis properties of the Terfezia claveryi truffle were investigated. Solvent extractions of the T. claveryi were evaluated for antioxidant activities using (DPPH, FRAP and ABTS methods). The extracts cytotoxicity on the cancer cell lines (HT29, MCF-7, PC3 and U-87 MG) was determined by MTT assay, while the anti-angiogenic efficacy was tested using ex-vivo assay. All extracts showed moderate anticancer activities against all cancer cells ( p < 0.05). The hexane extract inhibited the brain cell line (U-87 MG) with an IC 50 of 50 μg/ml and significantly promoted cell apoptosis through the mitochondrial pathway and DNA fragmentation p < 0.001. The ethanol extract demonstrated potent antioxidants; DPPH, FRAP, and ABTS with an IC 50 value of 52, 48.5 and 64.7 μg/ml, respectively. In addition, the hexane and ethyl acetate extract significantly ( p < 0.001) inhibited the sprouting of microvessels by 100% and 81.2%, at 100 μg/ml, respectively. The GC analysis of the most active extract (hexane) showed the presence of several potent phytochemicals such as stigmasterol, beta-Sitosterol, squalene, lupeol, octadecadienoic acid, and oleic acid. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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