Effects of L-DOPA Pretreatment on the Kinetics, Migration and Carbon Ion Radiation Response of T98G Cells.

Autor:	Facoetti A; Fondazione CNAO, Pavia, Italy Facoetti@cnao.it., Cavagnini M; Fondazione CNAO, Pavia, Italy., Ciocca M; Fondazione CNAO, Pavia, Italy., Nano R; Department of Biology and Biotechnology 'L. Spallanzani', University of Pavia, Pavia, Italy., Pasi F; Oncological Radiotherapy Unit, IRCCS Fondazione Policlinico San Matteo, Pavia, Italy., Aprile C; Fondazione CNAO, Pavia, Italy., Lodola L; Nuclear Medicine Unit, IRCCS Fondazione Policlinico San Matteo, Pavia, Italy., Persico MG; Nuclear Medicine Unit, IRCCS Fondazione Policlinico San Matteo, Pavia, Italy.; University School for Advanced Studies IUSS Pavia, Pavia, Italy., Marenco M; Nuclear Medicine Unit, IRCCS Fondazione Policlinico San Matteo, Pavia, Italy., Valvo F; Fondazione CNAO, Pavia, Italy., Orecchia R; Fondazione CNAO, Pavia, Italy.; IEO European Institute of Oncology IRCCS, Milan, Italy.
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2019 Jan; Vol. 39 (1), pp. 119-125.
DOI:	10.21873/anticanres.13087
Abstrakt:	Background/aim: Glioblastoma is the most malignant and widespread brain tumor in adults, with a rapid clinical course. Recently, it has been hypothesized that L-DOPA plays a role in the diagnosis and treatment of glioblastoma. The aim of this study was to assess the effects of pretreatment with L-DOPA on the biological behavior of human T98G cells in vitro. Materials and Methods: T98G cells were treated with 50 μg/ml or 100 μg/ml of L-DOPA for 4 h and their morphology, growth rate, clonogenic survival and migratory capacity in basal conditions and after carbon ion irradiation were evaluated using standard methods. Results: Treated cells showed a lower growth rate and an increased migratory capacity that correlated with the dose of tested L-DOPA. Treatment with L-DOPA increased the growth rate of carbon ion irradiated T98G cells compared to control non-treated cells exposed to the same radiation dose. Conclusion: Our results open further questions about the overall advantage of L-DOPA treatment of glioblastoma. (Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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