Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas.

Autor: Shimizu T; Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Aichi, Japan.; Department of Physiological Sciences, School of Life Sciences, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan., Osanai Y; Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Aichi, Japan.; Department of Physiological Sciences, School of Life Sciences, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan., Tanaka KF; Department of Neuropsychiatry, School of Medicine, Keio University, Tokyo, Japan., Thai TQ; Departments of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi, Japan., Abe M; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan., Natsume R; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan., Sakimura K; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan., Ikenaka K; Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Aichi, Japan.; Department of Physiological Sciences, School of Life Sciences, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan.
Jazyk: angličtina
Zdroj: Journal of neurochemistry [J Neurochem] 2019 Jul; Vol. 150 (2), pp. 158-172. Date of Electronic Publication: 2019 Jan 29.
DOI: 10.1111/jnc.14657
Abstrakt: Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimulation can be transduced into intracellular biochemical signals through mechanosensors, such as integrin, p130Cas, talin and vinculin. However, the molecular mechanisms underlying the mechanical regulation of OLs by mechanosensors remain largely unknown. We found that morphology of OL was affected by knockdown of the mechanosensors p130Cas or talin1. Stretching of OL precursor cells induced the phosphorylation of p130Cas and talin-associated assembly of vinculin. Shear stress decreased the number of OL processes, whereas these effects were mechanically suppressed by dominant-negative (DN) p130Cas, but not by DN-talin1. To investigate the roles of p130Cas in post-natal OLs in vivo, we constructed a novel p130Cas knock-in mouse and found overexpression of p130Cas in vivo affected the number of mature OLs in the cortex. These results indicate that the mechanosensor p130Cas controls both OL morphogenesis and maturation.
(© 2018 International Society for Neurochemistry.)
Databáze: MEDLINE
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