Transcriptional profiling of the mutualistic bacterium Vibrio fischeri and an hfq mutant under modeled microgravity.

Autor:	Duscher AA; Department of Microbiology and Cell Science University of Florida, Space Life Science Lab, Merritt Island, FL 32953 USA., Conesa A; 2Department of Microbiology and Cell Science Institute of Food and Agricultural Research, Genetics Institute, University of Florida, Gainesville, FL 32611 USA., Bishop M; Department of Microbiology and Cell Science University of Florida, Space Life Science Lab, Merritt Island, FL 32953 USA., Vroom MM; Department of Microbiology and Cell Science University of Florida, Space Life Science Lab, Merritt Island, FL 32953 USA., Zubizarreta SD; Department of Microbiology and Cell Science University of Florida, Space Life Science Lab, Merritt Island, FL 32953 USA., Foster JS; Department of Microbiology and Cell Science University of Florida, Space Life Science Lab, Merritt Island, FL 32953 USA.
Jazyk:	angličtina
Zdroj:	NPJ microgravity [NPJ Microgravity] 2018 Dec 18; Vol. 4, pp. 25. Date of Electronic Publication: 2018 Dec 18 (Print Publication: 2018).
DOI:	10.1038/s41526-018-0060-1
Abstrakt:	For long-duration space missions, it is critical to maintain health-associated homeostasis between astronauts and their microbiome. To achieve this goal it is important to more fully understand the host-symbiont relationship under the physiological stress conditions of spaceflight. To address this issue we examined the impact of a spaceflight analog, low-shear-modeled microgravity (LSMMG), on the transcriptome of the mutualistic bacterium Vibrio fischeri . Cultures of V. fischeri and a mutant defective in the global regulator Hfq (∆ hfq ) were exposed to either LSMMG or gravity conditions for 12 h (exponential growth) and 24 h (stationary phase growth). Comparative transcriptomic analysis revealed few to no significant differentially expressed genes between gravity and the LSMMG conditions in the wild type or mutant V. fischeri at exponential or stationary phase. There was, however, a pronounced change in transcriptomic profiles during the transition between exponential and stationary phase growth in both V. fischeri cultures including an overall decrease in gene expression associated with translational activity and an increase in stress response. There were also several upregulated stress genes specific to the LSMMG condition during the transition to stationary phase growth. The ∆ hfq mutants exhibited a distinctive transcriptome profile with a significant increase in transcripts associated with flagellar synthesis and transcriptional regulators under LSMMG conditions compared to gravity controls. These results indicate the loss of Hfq significantly influences gene expression under LSMMG conditions in a bacterial symbiont. Together, these results improve our understanding of the mechanisms by which microgravity alters the physiology of beneficial host-associated microbes. Competing Interests: The authors declare no competing interests.
Databáze:	MEDLINE
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