Chitosan disrupts biofilm formation and promotes biofilm eradication in Staphylococcus species isolated from bovine mastitis.

Autor: Felipe V; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional Villa María (UNVM), Villa María, Argentina; Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional Villa María (UNVM), Villa María, Argentina., Breser ML; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional Villa María (UNVM), Villa María, Argentina; Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional Villa María (UNVM), Villa María, Argentina., Bohl LP; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional Villa María (UNVM), Villa María, Argentina; Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional Villa María (UNVM), Villa María, Argentina., Rodrigues da Silva E; Universidade Federal Rural de Pernambuco, Garanhuns, Brazil., Morgante CA; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional Villa María (UNVM), Villa María, Argentina., Correa SG; Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI)-Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina., Porporatto C; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional Villa María (UNVM), Villa María, Argentina; Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional Villa María (UNVM), Villa María, Argentina. Electronic address: cporporatto@unvm.edu.ar.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Apr 01; Vol. 126, pp. 60-67. Date of Electronic Publication: 2018 Dec 23.
DOI: 10.1016/j.ijbiomac.2018.12.159
Abstrakt: Staphylococci are the main pathogens associated with hard-to-control intramammary infections in dairy cattle, and bacterial biofilms are suspected to be responsible for the antimicrobial resistance and persistence of this disease. Biofilms have the ability to resist to higher levels of antibiotics and reduce their efficacy. It is thus necessary to develop strategies targeted to bacterial biofilm infections. Chitosan is a polysaccharide with a proven broad spectrum of antimicrobial activity against fungi and bacteria. The aim of this study was assess the effect of low molecular weight (LMW) chitosan against biofilm hyperproducer Staphylococcus spp. (S. aureus and S. xylosus) strains usually involved in chronic bovine mastitis, and to test their efficacy in biofilm formation and eradication. The results obtained showed that LMW chitosan is able to inhibit S. aureus and S. xylosus planktonic growth in a dose-dependent manner and reduce bacterial viability. LMW chitosan inhibits biofilm formation, reduces biofilm viability and disrupts established biofilm. These results indicate the inhibitory effects of chitosan on biofilm formation, and these effects are observed at lower concentrations for S. xylosus. Our studies show the potential of this biopolymer to be used as an effective antibiofilm agent able to act upon staphylococcal infections.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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