| Abstrakt: |
The regularities of formation and appearance of mutants after transfer or plating yeast cells on selective media are analysed. The knowledge of these patterns is necessary for quantitative evaluation of mutants' content in the culture. Mutants formed in a cell culture, prior to transfer onto selective medium, are revealed on this medium as colonies and form a wave in time that is being observed during several days. Due to residual growth of cells, new mutants are formed and revealed as the second wave. Following two waves, an increasing front may approach which is formed from mutants of unknown origin. For evaluation of the number of mutants in the culture, prior to transfer onto selective medium, it is necessary to count up only the "first wave" of mutant colonies' appearance. |
