Association between MTHFR 677C>T Polymorphism and Vitamin B12 Deficiency: A Case-control Study.

Autor: Al-Batayneh KM; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan., Zoubi MSA; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan.; Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan., Shehab M; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan., Al-Trad B; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan., Bodoor K; Department of Applied Biology, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan., Khateeb WA; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan., Aljabali AAA; Faculty of Pharmacy, Yarmouk University, Irbid, Jordan., Hamad MA; Department of Pathology, Faculty of Medicine, University of Dammam, Dammam, Kingdom of Saudi Arabia., Eaton G; Department of Biology, Rowan University, New Jersey, USA.
Jazyk: angličtina
Zdroj: Journal of medical biochemistry [J Med Biochem] 2018 Apr 01; Vol. 37 (2), pp. 141-147. Date of Electronic Publication: 2018 Apr 01 (Print Publication: 2018).
DOI: 10.1515/jomb-2017-0051
Abstrakt: Background: Vitamin B12 (cobalamin) deficiency is a prevalent worldwide health concern. Several factors are associated with vitamin B12 deficiency including lifestyle, genetic predisposition, and malfunctions in the absorption and transport of vitamin B12. In the current case-control study, we aimed at investigating the association between MTHFR polymorphisms and vitamin B12 deficiency in a Jordanian population.
Methods: Two polymorphic sites of the MTHFR gene (c.677C>T, rs1801133 and c.1286A>C, rs1801131) were analyzed using RFLP and DNA sequencing in a group of vitamin B12 deficient individuals (45 males and 55 females). As a control, 100 matching individuals (age and sex) with vitamin B12 levels > 200 ng/mL were also recruited for this study.
Results: The MTHFR c.677C>T variant was significantly associated with vitamin B12 deficiency in individuals from northern Jordan. The frequency of the homozygous MTHFR c.677C>T genotype was significantly higher in B12 deficient individuals in comparison with the control group (X 2 = 8.397, p = 0.0150). The T allele frequency showed significant association with vitamin B12 deficiency in the study population (OR= 1.684, 95% CI: 1.116 to 2.542, p = 0.017). On the other hand, the MTHFR c.1286A>C variant did not show significant association with vitamin B12 deficiency in the selected population.
Conclusions: Our results showed a significant association between homozygous MTHFR c.677C>T variant and T allele frequencies and vitamin B12 deficiency in the Jordanian population.
Competing Interests: Conflict of interest statement The authors stated that they have no conflicts of interest regarding the publication of this article.
Databáze: MEDLINE
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