A Surface-Induced Asymmetric Program Promotes Tissue Colonization by Pseudomonas aeruginosa.

Autor:	Laventie BJ; Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland., Sangermani M; Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland., Estermann F; Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland., Manfredi P; Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland., Planes R; IPBS - UMR 5089, Toulouse, France., Hug I; Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland., Jaeger T; Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland., Meunier E; IPBS - UMR 5089, Toulouse, France., Broz P; Department of Biochemistry, University of Lausanne, Lausanne, Switzerland., Jenal U; Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland. Electronic address: urs.jenal@unibas.ch.
Jazyk:	angličtina
Zdroj:	Cell host & microbe [Cell Host Microbe] 2019 Jan 09; Vol. 25 (1), pp. 140-152.e6. Date of Electronic Publication: 2018 Dec 20.
DOI:	10.1016/j.chom.2018.11.008
Abstrakt:	The opportunistic human pathogen Pseudomonas aeruginosa effectively colonizes host epithelia using pili as primary adhesins. Here we uncover a surface-specific asymmetric virulence program that enhances P. aeruginosa host colonization. We show that when P. aeruginosa encounters surfaces, the concentration of the second messenger c-di-GMP increases within a few seconds. This leads to surface adherence and virulence induction by stimulating pili assembly through activation of the c-di-GMP receptor FimW. Surface-attached bacteria divide asymmetrically to generate a piliated, surface-committed progeny (striker) and a flagellated, motile offspring that leaves the surface to colonize distant sites (spreader). Cell differentiation is driven by a phosphodiesterase that asymmetrically positions to the flagellated pole, thereby maintaining c-di-GMP levels low in the motile offspring. Infection experiments demonstrate that cellular asymmetry strongly boosts infection spread and tissue damage. Thus, P. aeruginosa promotes surface colonization and infection transmission through a cooperative virulence program that we termed Touch-Seed-and-Go. (Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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