Synthetic anionic surfaces can replace microparticles in stimulating burst coagulation of blood plasma.

Autor: Contreras-García A; Groupe de Physique et Technologie des Couches Minces (GCM), Department of Engineering Physics, École Polytechnique de Montréal, Montreal, QC, H3C 3A7, Canada., D'Elía NL; Department of Chemical Engineering, École Polytechnique de Montréal, Montreal, QC, H3C 3A7, Canada; Department of Chemistry, Universidad Nacional del Sur, CONICET, 8000, Bahia Blanca, Argentina., Desgagné M; Department of Chemical Engineering, École Polytechnique de Montréal, Montreal, QC, H3C 3A7, Canada., Lafantaisie-Favreau CH; Department of Chemical Engineering, École Polytechnique de Montréal, Montreal, QC, H3C 3A7, Canada., Rivard GE; Hematology Oncology, CHU Sainte-Justine, Montreal, QC, H3T 1C5, Canada., Ruiz JC; División de Ciencias Básicas e Ingeniería, Depto. de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, 09340 México D.F., Mexico., Wertheimer MR; Groupe de Physique et Technologie des Couches Minces (GCM), Department of Engineering Physics, École Polytechnique de Montréal, Montreal, QC, H3C 3A7, Canada; Institute of Biomedical Engineering, École Polytechnique de Montréal, C.P. 6079, Succursale Centre-Ville, Montréal, QC, H3C 3A7, Canada., Messina P; Department of Chemistry, Universidad Nacional del Sur, CONICET, 8000, Bahia Blanca, Argentina., Hoemann CD; Department of Chemical Engineering, École Polytechnique de Montréal, Montreal, QC, H3C 3A7, Canada; Institute of Biomedical Engineering, École Polytechnique de Montréal, C.P. 6079, Succursale Centre-Ville, Montréal, QC, H3C 3A7, Canada; Department of Bioengineering, George Mason University, Manassas, VA, USA. Electronic address: choemann@gmu.edu.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2019 Mar 01; Vol. 175, pp. 596-605. Date of Electronic Publication: 2018 Nov 29.
DOI: 10.1016/j.colsurfb.2018.11.066
Abstrakt: Biomaterials are frequently evaluated for pro-coagulant activity but usually in the presence of microparticles (MPs), cell-derived vesicles in blood plasma whose phospholipid surfaces allow coagulation factors to set up as functional assemblies. We tested the hypothesis that synthetic anionic surfaces can catalyze burst thrombin activation in human blood plasma in the absence of MPs. In a thromboelastography (TEG) assay with plastic sample cups and pins, recalcified human citrated platelet-poor plasma spontaneously burst-coagulated but with an unpredictable clotting time whereas plasma depleted of MPs by ultracentrifugation failed to coagulate. Coagulation of MP-depleted plasma was restored in a dose-dependent manner by glass microbeads, hydroxyapatite nanoparticles (HA NPs), and carboxylic acid-containing anionic nanocoatings of TEG cups and pins (coated by glow-discharge plasma-polymerized ethylene containing oxygen, L-PPE:O with 4.4 and 6.8 atomic % [COOH]). Glass beads lost their pro-coagulant activity in MP-depleted plasma after their surfaces were nanocoated with hydrophobic plasma-polymerized hexamethyl disiloxane (PP-HMDSO). In FXII-depleted MP-depleted plasma, glass microbeads failed to induce coagulation, however, FXIa was sufficient to induce coagulation in a dose-dependent manner, with no effect of glass beads. These data suggest that anionic surfaces of crystalline, organic, and amorphous solid synthetic materials catalyze explosive thrombin generation in MP-depleted plasma by activating the FXII-dependent intrinsic contact pathway. The data also show that microparticles are pro-coagulant surfaces whose activity has been largely overlooked in many coagulation studies to-date. These results suggest a possible mechanism by which anionic biomaterial surfaces induce bone healing by contact osteogenesis, through fibrin clot formation in the absence of platelet activation.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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