Inactivation of the bacterial pathogens Staphylococcus pseudintermedius and Acinetobacter baumannii by butanoic acid.

Autor: Kennedy GM; Department of Chemistry and Life Science, United States Military Academy, West Point, NY, USA., Min MY; Department of Chemistry and Life Science, United States Military Academy, West Point, NY, USA., Fitzgerald JF; Department of Chemistry and Life Science, United States Military Academy, West Point, NY, USA., Nguyen MT; Department of Geography and Environmental Engineering, United States Military Academy, West Point, NY, USA., Schultz SL; Department of Biology, University of New England, Biddeford, ME, USA., Crum MT; Department of Chemistry and Life Science, United States Military Academy, West Point, NY, USA., Starke JA; Department of Geography and Environmental Engineering, United States Military Academy, West Point, NY, USA., Butkus MA; Department of Geography and Environmental Engineering, United States Military Academy, West Point, NY, USA., Bowman DD; Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY, USA., Labare MP; Department of Chemistry and Life Science, United States Military Academy, West Point, NY, USA.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2019 Mar; Vol. 126 (3), pp. 752-763. Date of Electronic Publication: 2019 Jan 31.
DOI: 10.1111/jam.14180
Abstrakt: Aims: This study was performed to evaluate the efficacy of butanoic acid against bacterial pathogens including Acinetobacter baumannii and Staphylococcus pseudintermedius.
Methods and Results: Vegetative bacteria were exposed to butanoic acid in vitro and log reduction was quantified using viable count assays. The maximum (8 and 9) log inactivation was determined by qualitatively assaying for growth/no-growth after a 48-h incubation (37°C). Membrane integrity after exposure to butanoic acid was determined by propidium iodide staining, scanning electron microscopy, membrane depolarization and inductively coupled plasma analysis. Cytosolic pH was measured by 5-(6-)carboxyfluorescein succinimidyl ester.
Conclusions: Inhibitory concentrations of butanoic acid ranged between 11 and 21 mmol l -1 for Gram-positive and Gram-negative species tested. The maximum log reduction of A. baumannii was achieved with a 10-s exposure of 0·50 mol l -1 of butanoic acid. Staphylococcus pseudintermedius required 0·40 mol l -1 of butanoic acid to achieve the same level of reduction in the same time period. Inactivation was associated with membrane permeability and acidification of the cytosol.
Significance and Impact of the Study: Antibiotic resistance among bacterial pathogens necessitates the utilization of novel therapeutics for disinfection and biological control. These results may facilitate the development of butanoic acid as an effective agent against a broad-spectrum of antibiotic-resistant bacterial pathogens.
(© 2018 The Society for Applied Microbiology.)
Databáze: MEDLINE
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