Alcohol extract of Bauhinia forficata link reduces lipid peroxidation in the testis and epididymis of adult Wistar rats.

Autor: Sampaio CF; General Biology Department, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., Lucchetta NR; General Biology Department, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil.; Pharmaceutical Sciences Department, Health Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., Punhagui APF; General Biology Department, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil.; General Pathology Department, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., Banedetti PR; Biology and Technology Department, State University of North Paraná - UENP, Bandeirantes, Paraná, Brazil., Arakawa NS; Pharmaceutical Sciences Department, Health Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil., Seiva FRF; Biology and Technology Department, State University of North Paraná - UENP, Bandeirantes, Paraná, Brazil., Fernandes GSA; General Biology Department, Biological Sciences Center, State University of Londrina - UEL, Londrina, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Microscopy research and technique [Microsc Res Tech] 2019 Apr; Vol. 82 (4), pp. 345-351. Date of Electronic Publication: 2018 Dec 21.
DOI: 10.1002/jemt.23175
Abstrakt: Bauhinia forficata is a medicinal plant that has flavonoid components with hypoglycemic, antioxidant, hepatoprotective, antibacterial, antiviral and anti-inflammatory action. Aim of this study is to evaluate the action of B. forficata alcoholic extract in the male genital system of adult male Wistar rats. For that, 20 adult male Wistar rats were distributed into two experimental groups: the B. forficata group, receiving B. forficata alcoholic extract (0.1 ml/10 g body weight/day) on alternate days, and the control group, receiving just the vehicle for 30 days straight both via gavage. On the 31st day, the animals were euthanized, and the testis and epididymis were collected for histopathological, biochemical, morphometric, and sperm count analysis. Mass spectrometry identified new compounds in the extract: trans-caffeic acid, liquiritigenin, gallocatechin, and 2,4,6-trihydroxyphenanthren-2-glycoside. Biochemical analysis showed higher total cholesterol levels in the testis and lower malondialdehyde levels in the testis and epididymis, in the B. forficata group. The mast cell count showed a reduction in degranulated mast cells in the caput region of the epididymis, in the B. forficata group. The luminal compartment of the caput and the epithelial of the epididymis cauda were reduced, whereas the stromal region of the epididymis caput was increased in the B. forficata group, compared with the control group. The testicular tissue was less impaired, considering that all the histological analyses were similar to the control. We believe that B. forficata alcoholic extract in the male genital system showed antioxidant action, especially in the epididymal tissue.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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