Methods for detecting Zika virus in feces: A case study in captive squirrel monkeys (Saimiri boliviensis boliviensis).

Autor: Milich KM; Department of Anthropology, Washington University in St. Louis, St. Louis, Missouri, United States of America.; Department of Anthropology, University of Texas at Austin, Austin, Texas, United States of America., Koestler BJ; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology and LaMontagne Center for Infectious Disease, University of Texas at Austin, Austin, Texas, United States of America., Simmons JH; University of Texas MD Anderson Cancer Research Center, Bastrop, Texas, United States of America., Nehete PN; University of Texas MD Anderson Cancer Research Center, Bastrop, Texas, United States of America., Di Fiore A; Department of Anthropology, University of Texas at Austin, Austin, Texas, United States of America., Williams LE; University of Texas MD Anderson Cancer Research Center, Bastrop, Texas, United States of America., Dudley JP; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology and LaMontagne Center for Infectious Disease, University of Texas at Austin, Austin, Texas, United States of America., Vanchiere J; Department of Pediatrics, Louisiana State University Health Science Center at Shreveport, Shreveport, Louisiana, United States of America., Payne SM; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology and LaMontagne Center for Infectious Disease, University of Texas at Austin, Austin, Texas, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Dec 20; Vol. 13 (12), pp. e0209391. Date of Electronic Publication: 2018 Dec 20 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0209391
Abstrakt: A strain of Zika virus (ZIKV) of Asian origin associated with birth defects and neurological disorders has emerged and spread through the Americas. ZIKV was first isolated in the blood of nonhuman primates in Africa and has been detected in the blood, saliva, and urine of a few catarrhine species in both Africa and Asia, suggesting that nonhuman primates may serve as both a source and a reservoir of the virus. The recent introduction of ZIKV to human populations in the Americas presents the potential for the virus to spread into nonhuman primate reservoirs. Thus, it is critical to develop efficient and noninvasive detection methods to monitor the spread of the virus in wild nonhuman primate populations. Here, we describe a method for ZIKV detection in noninvasively collected fecal samples of a Neotropical primate. Fecal samples were collected from two captive squirrel monkeys (Saimiri boliviensis boliviensis) that were experimentally infected with ZIKV (Strain Mexico_1_44) and an additional two uninfected squirrel monkeys. Nucleic acids were extracted from these samples, and RT-qPCR was used to assay for the presence of ZIKV using primers flanking a 101 bp region of the NS5 gene. In both ZIKV-inoculated animals, ZIKV was detected 5-11 days post-infection, but was not detected in the uninfected animals. We compare the fecal results to ZIKV detection in serum, saliva, and urine samples from the same individuals. Our results indicate that fecal detection is a cost-effective, noninvasive method for monitoring wild populations of Neotropical primates as possible ZIKV reservoirs.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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