Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish.

Autor:	Saju JM; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore., Hossain MS; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore; Department of Biological Sciences, National University of Singapore, Singapore, Singapore., Liew WC; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore., Pradhan A; Biology, The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden., Thevasagayam NM; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore., Tan LSE; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore., Anand A; Bioimaging and Biocomputing, Temasek Life Sciences Laboratory, Singapore, Singapore. Electronic address: amit@tll.org.sg., Olsson PE; Biology, The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden. Electronic address: per-erik.olsson@oru.se., Orbán L; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore; Frontline Fish Genomics Research Group, Department of Animal Sciences, Georgikon Faculty, University of Pannonia, Keszthely, Hungary; Centre for Comparative Genomics, Murdoch University, Murdoch, Australia. Electronic address: orban@georgikon.hu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2018 Dec 18; Vol. 25 (12), pp. 3252-3261.e4.
DOI:	10.1016/j.celrep.2018.11.090
Abstrakt:	Heat shock factors (Hsfs) are transcription factors that regulate responses to heat shock and other environmental stimuli. Four heat shock factors (Hsf1-4) have been characterized from vertebrates to date. In addition to stress response, they also play important roles in development and gametogenesis. Here, we study the fifth member of heat shock factor family, Hsf5, using zebrafish as a model organism. Mutant hsf5 -/- males, generated by CRISPR/Cas9 technique, were infertile with drastically reduced sperm count, increased sperm head size, and abnormal tail architecture, whereas females remained fertile. We show that Hsf5 is required for progression through meiotic prophase 1 during spermatogenesis as suggested by the accumulation of cells in the leptotene and zygotene-pachytene stages and increased apoptosis in post-meiotic cells. hsf5 -/- mutants show gonadal misregulation of a substantial number of genes with roles in cell cycle, apoptosis, protein modifications, and signal transduction, indicating an important role of Hsf5 in early stages of spermatogenesis. (Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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