NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system.

Autor: French CD; Department of Nutrition, University of California, Davis, California, United States of America., Willoughby RE; Department of Pediatrics, Division of Infectious Disease, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America., Pan A; Department of Pediatrics, Division of Infectious Disease, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America., Wong SJ; Wadsworth Center Diagnostic Immunology Laboratory, New York State Department of Health, Albany, New York, United States of America., Foley JF; Intermountain Healthcare, Salt Lake City, Utah, United States of America., Wheat LJ; Department of Medicine, Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana, United States of America., Fernandez J; Hospital Infantil Robert Reid Cabral, Santo Domingo, Distrito Nacional, República Dominicana., Encarnacion R; Hospital Infantil Robert Reid Cabral, Santo Domingo, Distrito Nacional, República Dominicana., Ondrush JM; Inova Fairfax Hospital, Fairfax, Virginia, United States of America., Fatteh N; Inova Fairfax Hospital, Fairfax, Virginia, United States of America., Paez A; Departamento de Ciencias Basicas, Universidad de la Salle, Bogotá, Colombia., David D; Rabies Lab, Kimron Veterinary Institute, Beit Dagan, Israel., Javaid W; Department of Medicine, SUNY Upstate Medical University, Syracuse, New York, United States of America., Amzuta IG; Department of Medicine, SUNY Upstate Medical University, Syracuse, New York, United States of America., Neilan AM; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America., Robbins GK; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America., Brunner AM; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America., Hu WT; Mayo Clinic, Rochester, Minnesota, United States of America., Mishchuk DO; Department of Food Science and Technology, University of California, Davis, California, United States of America., Slupsky CM; Department of Nutrition, University of California, Davis, California, United States of America.; Department of Food Science and Technology, University of California, Davis, California, United States of America.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2018 Dec 17; Vol. 12 (12), pp. e0007045. Date of Electronic Publication: 2018 Dec 17 (Print Publication: 2018).
DOI: 10.1371/journal.pntd.0007045
Abstrakt: Background: Myriad infectious and noninfectious causes of encephalomyelitis (EM) have similar clinical manifestations, presenting serious challenges to diagnosis and treatment. Metabolomics of cerebrospinal fluid (CSF) was explored as a method of differentiating among neurological diseases causing EM using a single CSF sample.
Methodology/principal Findings: 1H NMR metabolomics was applied to CSF samples from 27 patients with a laboratory-confirmed disease, including Lyme disease or West Nile Virus meningoencephalitis, multiple sclerosis, rabies, or Histoplasma meningitis, and 25 controls. Cluster analyses distinguished samples by infection status and moderately by pathogen, with shared and differentiating metabolite patterns observed among diseases. CART analysis predicted infection status with 100% sensitivity and 93% specificity.
Conclusions/significance: These preliminary results suggest the potential utility of CSF metabolomics as a rapid screening test to enhance diagnostic accuracies and improve patient outcomes.
Competing Interests: The authors have declared that no competing interests exist
Databáze: MEDLINE
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