Self-Assembly of a Designed Nucleoprotein Architecture through Multimodal Interactions.

Autor:	Subramanian RH; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States., Smith SJ; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States., Alberstein RG; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States., Bailey JB; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States., Zhang L; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States., Cardone G; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States., Suominen L; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States., Chami M; C-CINA, Biozentrum, University of Basel, Mattenstrasse 26, 4058 Basel, Switzerland., Stahlberg H; C-CINA, Biozentrum, University of Basel, Mattenstrasse 26, 4058 Basel, Switzerland., Baker TS; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.; Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, United States., Tezcan FA; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.; Materials Science and Engineering, University of California, San Diego, La Jolla, California 92093, United States.
Jazyk:	angličtina
Zdroj:	ACS central science [ACS Cent Sci] 2018 Nov 28; Vol. 4 (11), pp. 1578-1586. Date of Electronic Publication: 2018 Nov 15.
DOI:	10.1021/acscentsci.8b00745
Abstrakt:	The co-self-assembly of proteins and nucleic acids (NAs) produces complex biomolecular machines (e.g., ribosomes and telomerases) that represent some of the most daunting targets for biomolecular design. Despite significant advances in protein and DNA or RNA nanotechnology, the construction of artificial nucleoprotein complexes has largely been limited to cases that rely on the NA-mediated spatial organization of protein units, rather than a cooperative interplay between protein- and NA-mediated interactions that typify natural nucleoprotein assemblies. We report here a structurally well-defined synthetic nucleoprotein assembly that forms through the synergy of three types of intermolecular interactions: Watson-Crick base pairing, NA-protein interactions, and protein-metal coordination. The fine thermodynamic balance between these interactions enables the formation of a crystalline architecture under highly specific conditions. Competing Interests: The authors declare no competing financial interest.
Databáze:	MEDLINE
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