House dust mite drives proinflammatory eicosanoid reprogramming and macrophage effector functions.
| Autor: | Henkel FDR; Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Munich, Germany., Friedl A; Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Munich, Germany., Haid M; Institute of Experimental Genetics, Genome Analysis Center, Neuherberg, Germany., Thomas D; Pharmazentrum frankfurt/ZAFES, Institute of Clinical Pharmacology, Goethe University Frankfurt, Frankfurt, Germany., Bouchery T; Laboratory of Intestinal Immunology, Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Department of Immunology and Pathology, Central Clinical School, Alfred Medical Research and Education Precinct, Monash University, Clayton, Victoria, Australia., Haimerl P; Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Munich, Germany., de Los Reyes Jiménez M; Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Munich, Germany., Alessandrini F; Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Munich, Germany., Schmidt-Weber CB; Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Munich, Germany., Harris NL; Laboratory of Intestinal Immunology, Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Department of Immunology and Pathology, Central Clinical School, Alfred Medical Research and Education Precinct, Monash University, Clayton, Victoria, Australia., Adamski J; Institute of Experimental Genetics, Genome Analysis Center, Neuherberg, Germany.; Lehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan, Germany., Esser-von Bieren J; Center of Allergy and Environment (ZAUM), Technical University of Munich and Helmholtz Center Munich, Munich, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Allergy [Allergy] 2019 Jun; Vol. 74 (6), pp. 1090-1101. Date of Electronic Publication: 2019 Jan 08. |
| DOI: | 10.1111/all.13700 |
| Abstrakt: | Background: Eicosanoid lipid mediators play key roles in type 2 immune responses, for example in allergy and asthma. Macrophages represent major producers of eicosanoids and they are key effector cells of type 2 immunity. We aimed to comprehensively track eicosanoid profiles during type 2 immune responses to house dust mite (HDM) or helminth infection and to identify mechanisms and functions of eicosanoid reprogramming in human macrophages. Methods: We established an LC-MS/MS workflow for the quantification of 52 oxylipins to analyze mediator profiles in human monocyte-derived macrophages (MDM) stimulated with HDM and during allergic airway inflammation (AAI) or nematode infection in mice. Expression of eicosanoid enzymes was studied by qPCR and western blot and cytokine production was assessed by multiplex assays. Results: Short (24 h) exposure of alveolar-like MDM (aMDM) to HDM suppressed 5-LOX expression and product formation, while triggering prostanoid (thromboxane and prostaglandin D Conclusion: Our findings show that a short exposure to allergens as well as ongoing type 2 immune responses are characterized by a fundamental reprogramming of the lipid mediator metabolism with macrophages representing particularly plastic responder cells. Targeting mediator reprogramming in airway macrophages may represent a viable approach to prevent pathogenic lipid mediator profiles in allergy or asthma. (© 2018 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.) |
| Databáze: | MEDLINE |
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