The role of E-cadherin and Runx3 in Helicobacter Pylori - Associated gastric carcinoma is achieved through regulating P21waf and P27 expression.

Autor: Bahnassy AA; Tissue Culture and Cytogenetics Unit, Pathology Department, NCI, Cairo University, Egypt. Electronic address: chaya2000@hotmail.com., Helal TE; Pathology Department, Faculty of Medicine, Ain Shams University, Egypt., El-Ghazawy IM; Surgery Department, Faculty of Medicine, Ain Shams University, Egypt., Samaan GF; Clinical Pathology Department, Mataryia Teaching Hospital, Cairo, Egypt., Galal El-Din MM; Surgery Department, Mataryia Teaching Hospital, Egypt., Abdellateif MS; Molecular Virology and Immunology Unit, Cancer Biology Department, NCI, Cairo University, Egypt. Electronic address: mona.sayed@nci.cu.edu.eg., Desouky E; Epidemiology and Biostatistics Department, NCI, Cairo University, Egypt., Zekri AN; Molecular Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Cancer genetics [Cancer Genet] 2018 Dec; Vol. 228-229, pp. 64-72. Date of Electronic Publication: 2018 Sep 19.
DOI: 10.1016/j.cancergen.2018.08.006
Abstrakt: Background: We assessed the role of E-cadherin (CDH1), runt-related transcription factor 3, p21waf and p27 promoter methylation (PM) and protein expression in Helicobacter pylori (HP)-associated gastric carcinomas (GCs) and adjacent non-neoplastic tissues (ANNTs).
Patients and Methods: 192 cases were assessed for PM and protein expression of CDH1, RUNX3, p21waf and p27 by methylation-specific PCR (MSP) and immunohistochemistry. The CagA gene was also assessed.
Results: In GCs, 66 (34.4%) and 84 (43.8%) cases showed CDH1-PM and reduced expression. It is significantly affected in GCs rather than in non-neoplastic groups (p < 0.001). In ANNTs, 108 (56.3%) cases showed CDH1-PM and all cases revealed preserved protein expression. RUNX3-PM was detected in 78 GCs (40.6%) and 69 ANNTs (35.9%), whereas reduced protein expression was detected in 99 (51.65%) GC compared to ANNTs 90 (46.9%). p21WAF and p27 showed PM in (48.4% and 45.3%) GCs and ANNTs; respectively. p21waf protein was reduced in 90 (46.9%) cases and 91 ANNTs (47.4%). p27 was reduced in 86 (44.8%) cases and 87 ANNTs (45.3%). CDH1 aberrations correlated with HP in tumors and ANNTs and with diffuse/intestinal tumors (p = 0.014, p = 0.014 and p = 0.02). RUNX3 aberrations associated with HP (p = 0.04), high grade (p = 0.04), and advanced stage (p = 032). Tumor grade associated with RUNX3-PM, CDH, p21 and p27 protein (p < 0.05 for all). Tumor stage associated significantly with PM and reduced protein expression of all markers. Positive lymph nodes associated significantly with p27PM (p < 0.001).
Conclusions: HP plays an important role in the development and progression of GC through silencing of CDH1, RUNX3, p21WAF and p27 expression.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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