Induction of protective immunity by recombinant peptidoglycan associated lipoprotein (rPAL) protein of Legionella pneumophila in a BALB/c mouse model.

Autor: Mobarez AM; Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: mmmobarez@modares.ac.ir., Rajabi RA; Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Salmanian AH; Department of Plant Biotechnology, National Institutes for Genetic Engineering and Biotechnology, Tehran, Iran., Khoramabadi N; Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran., Hosseini Doust SR; Department of Microbiology, Faculty of Advanced Sciences, Medical Sciences University Islamic Azad, Tehran, Iran.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2019 Mar; Vol. 128, pp. 100-105. Date of Electronic Publication: 2018 Dec 11.
DOI: 10.1016/j.micpath.2018.12.014
Abstrakt: Legionella pneumophila causes a severe form of pneumonia known as Legionnaires' disease especially in patients with impaired cellular immune response. In order to prevent the disease, immunogenicity and the level of the induction of protective immunity from the recombinant peptidoglycan-associated lipoprotein (rPAL) against Legionella pneumophila in BALB/c mice was examined. Mice immunized with (rPAL) rapidly increased an antibody response in serum and also displayed a strong activation of both innate and adaptive cell-mediated immunity as determined by antigen-specific splenocyte proliferation, an early production of pro-inflammatory cytokines in the serum and in the splenocyte cultures. Infection with a primary sublethal does of Legionella pneumophila serogroup 1, strain paris, caused resistance to a lethal challenge infection in the animals with 100% survival rate. However, mice treated with rPAL survived with 60% rate in 10 days after a lethal i.v challenge with L. pneumophila. All of the control animals receiving PBS died within 24 h. The present study indicates that recombinant protein PAL of Legionella pneumophila is strongly immunogenic and capable to elicit early innate and adaptive immune responses and lasting immunity against a lethal dose of Legionella pneumophila challenge. Antigenic characterization and immune protection of recombinant protein PAL would be of considerable value in comprehension the immune-pathogenesis of the disease and in development possible vaccine against the Legionella.
(Copyright © 2018. Published by Elsevier Ltd.)
Databáze: MEDLINE
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