Tenogenically differentiated adipose-derived stem cells are effective in Achilles tendon repair in vivo.

Autor: Norelli JB; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USA.; Orthopaedic Research Laboratory, The Feinstein Institute for Medical Research, Manhasset, NY, USA., Plaza DP; Orthopaedic Research Laboratory, The Feinstein Institute for Medical Research, Manhasset, NY, USA.; Drexel University College of Medicine, Philadelphia, PA, USA., Stal DN; Orthopaedic Research Laboratory, The Feinstein Institute for Medical Research, Manhasset, NY, USA.; Department of Orthopedic Surgery, Northwell Health, Manhasset, NY, USA., Varghese AM; Orthopaedic Research Laboratory, The Feinstein Institute for Medical Research, Manhasset, NY, USA., Liang H; Orthopaedic Research Laboratory, The Feinstein Institute for Medical Research, Manhasset, NY, USA.; Department of Orthopedic Surgery, Northwell Health, Manhasset, NY, USA., Grande DA; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USA.; Orthopaedic Research Laboratory, The Feinstein Institute for Medical Research, Manhasset, NY, USA.; Department of Orthopedic Surgery, Northwell Health, Manhasset, NY, USA.
Jazyk: angličtina
Zdroj: Journal of tissue engineering [J Tissue Eng] 2018 Nov 12; Vol. 9, pp. 2041731418811183. Date of Electronic Publication: 2018 Nov 12 (Print Publication: 2018).
DOI: 10.1177/2041731418811183
Abstrakt: The purpose of this study was to characterize rat adipose-derived stem cells, induce adipose-derived stem cell tenogenesis, and analyze adipose-derived stem cell effects on tendon repair in vivo. Adipose-derived stem cells demonstrated an immunomodulatory, pro-angiogenic, and pro-proliferatory profile in vitro. Tenogenesis was induced for 1, 7, 14, and 21 days with 24 combinations of growth differentiation factor-5, 6, and 7 and platelet-derived growth factor-BB. Adipose-derived stem cells expression of scleraxis and collagen type I increased the most after 14 days of induction with growth differentiation factor-6 and platelet-derived growth factor-BB. Achilles excision defects injected with hydrogel alone (Gp2), with undifferentiated (Gp3) adipose-derived stem cells, or tenogenically differentiated (Gp4) adipose-derived stem cells exhibited improved tissue repair compared with untreated tendons (Gp1). Addition of adipose-derived stem cells improved tissue cytoarchitecture and increased expression of collagen type I and III, scleraxis, and tenomodulin. Adipose-derived stem cells significantly improved biomechanical properties (ultimate load and elastic toughness) over time more than hydrogel alone, while tenogenically differentiated adipose-derived stem cells improved the mean histological score and collagen fiber dispersion range closest to normal tendon. In addition, tendon sections treated with GFP-adipose-derived stem cells exhibited green fluorescence and positive GFP immunostaining on microscopy confirming the in vivo survival of adipose-derived stem cells that were injected into tendon defects to support the effects of adipose-derived stem cells on tissue up to 4.5 weeks post injury.
Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze: MEDLINE
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