A Host ER Fusogen Is Recruited by Turnip Mosaic Virus for Maturation of Viral Replication Vesicles.
| Autor: | Movahed N; Department of Biology, McGill University, Montreal, Quebec, H3A 1B1, Canada., Sun J; Department of Biology, McGill University, Montreal, Quebec, H3A 1B1, Canada., Vali H; Facility for Electron Microscopy Research, McGill University, Montreal, Quebec, H3A 0C7, Canada.; Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, H3A 0C7, Canada., Laliberté JF; INRS-Institut Armand-Frappier, Laval, Quebec, H7V 1B7, Canada., Zheng H; Department of Biology, McGill University, Montreal, Quebec, H3A 1B1, Canada hugo.zheng@mcgill.ca. |
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| Jazyk: | angličtina |
| Zdroj: | Plant physiology [Plant Physiol] 2019 Feb; Vol. 179 (2), pp. 507-518. Date of Electronic Publication: 2018 Dec 11. |
| DOI: | 10.1104/pp.18.01342 |
| Abstrakt: | Like other positive-strand RNA viruses, the Turnip mosaic virus (TuMV) infection leads to the formation of viral vesicles at the endoplasmic reticulum (ER). Once released from the ER, the viral vesicles mature intracellularly and then move intercellularly. While it is known that the membrane-associated viral protein 6K2 plays a role in the process, the contribution of host proteins has been poorly defined. In this article, we show that 6K2 interacts with RHD3, an ER fusogen required for efficient ER fusion. When RHD3 is mutated, a delay in the development of TuMV infection is observed. We found that the replication of TuMV and the cell-to-cell movement of its replication vesicles are impaired in rhd3 This defect can be tracked to a delayed maturation of the viral vesicles from the replication incompetent to the competent state. Furthermore, 6K2 can relocate RHD3 from the ER to viral vesicles. However, a Golgi-localized mutated 6K2 GV is unable to interact and relocate RHD3 to viral vesicles. We conclude that the maturation of TuMV replication vesicles requires RHD3 for efficient viral replication and movement. (© 2019 American Society of Plant Biologists. All Rights Reserved.) |
| Databáze: | MEDLINE |
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