| Autor: |
Slavcev RA; University of Waterloo, School of Pharmacy, Waterloo, ON, Canada. slavcev@uwaterloo.ca., Sum CH; University of Waterloo, School of Pharmacy, Waterloo, ON, Canada., St Jean J; University of Waterloo, School of Pharmacy, Waterloo, ON, Canada., Huh H; University of Waterloo, School of Pharmacy, Waterloo, ON, Canada., Nafissi N; University of Waterloo, School of Pharmacy, Waterloo, ON, Canada. |
| Abstrakt: |
Fluorescent-based visualization techniques have long been used to monitor biological activity. This chapter explores the delivery of reporter genes as a means to assay and track activity in biological systems. Bioluminescence is the production of light due to biochemical processes. By encoding genes for bioluminescence, biological processes can be visualized based on gene expression. This chapter also discusses the primary applications of bioluminescence as seen through bioluminescent imaging techniques, flow cytometry, and PCR-based methods of gene detection. These techniques are described in terms of researching gene expression, cancer therapy, and protein interactions. |
