Identification and Isolation of Clonogenic Cholangiocyte in Mouse.

Autor:	Li B; Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR, USA. libi@ohsu.edu., Dorrell C; Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR, USA., Canady PS; Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR, USA., Wakefield L; Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1905, pp. 19-27.
DOI:	10.1007/978-1-4939-8961-4_3
Abstrakt:	Cholangiocytes are proliferative and are one of the sources for liver progenitor cells. Clonogenic cholangiocytes are defined as cells capable of clonally proliferating and differentiating cholangiocytes both in vitro and in vivo. In this protocol, we describe the method for isolation of primary cholangiocytes from mouse. To study the heterogeneity of cholangiocytes, we used flow cytometry-based cell sorting to isolate different subsets of cholangiocytes. Organoid-forming efficiencies from sorted single cells are compared within different cholangiocyte populations to identify clonogenic cholangiocytes.
Databáze:	MEDLINE
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