The role of roughage provision on the absorption and disposition of the mycotoxin deoxynivalenol and its acetylated derivatives in calves: from field observations to toxicokinetics.

Autor: Valgaeren B; Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.; Faculty of Science and Technology, University College Ghent, Melle, Belgium., Théron L; Clinical Department of Production Animals, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium., Croubels S; Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Devreese M; Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., De Baere S; Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Van Pamel E; Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit-Food Safety, Melle, Belgium., Daeseleire E; Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit-Food Safety, Melle, Belgium., De Boevre M; Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium., De Saeger S; Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium., Vidal A; Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium., Di Mavungu JD; Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium., Fruhmann P; Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria.; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Tulln, Austria., Adam G; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Tulln, Austria., Callebaut A; Veterinary and Agrochemical Research Centre, CODA-CERVA, Tervuren, Belgium., Bayrou C; Department of Pathology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium., Frisée V; Clinical Department of Production Animals, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium., Rao AS; Clinical Department of Production Animals, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium., Knapp E; Clinical Department of Production Animals, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium., Sartelet A; Clinical Department of Production Animals, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium., Pardon B; Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Deprez P; Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Antonissen G; Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Gunther.Antonissen@UGent.be.; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Gunther.Antonissen@UGent.be.
Jazyk: angličtina
Zdroj: Archives of toxicology [Arch Toxicol] 2019 Feb; Vol. 93 (2), pp. 293-310. Date of Electronic Publication: 2018 Dec 10.
DOI: 10.1007/s00204-018-2368-8
Abstrakt: A clinical case in Belgium demonstrated that feeding a feed concentrate containing considerable levels of deoxynivalenol (DON, 1.13 mg/kg feed) induced severe liver failure in 2- to 3-month-old beef calves. Symptoms disappeared by replacing the highly contaminated corn and by stimulating ruminal development via roughage administration. A multi-mycotoxin contamination was demonstrated in feed samples collected at 15 different veal farms in Belgium. DON was most prevalent, contaminating 80% of the roughage samples (mixed straw and maize silage; average concentration in positives: 637 ± 621 µg/kg, max. 1818 µg/kg), and all feed concentrate samples (411 ± 156 µg/kg, max. 693 µg/kg). In order to evaluate the impact of roughage provision and its associated ruminal development on the gastro-intestinal absorption and biodegradation of DON and its acetylated derivatives (3- and 15-ADON) in calves, a toxicokinetic study was performed with two ruminating and two non-ruminating male calves. Animals received in succession a bolus of DON (120 µg/kg bodyweight (BW)), 15-ADON (50 µg/kg BW), and 3-ADON (25 µg/kg) by intravenous (IV) injection or per os (PO) in a cross-over design. The absolute oral bioavailability of DON was much higher in non-ruminating calves (50.7 ± 33.0%) compared to ruminating calves (4.1 ± 4.5%). Immediately following exposure, 3- and 15-ADON were hydrolysed to DON in ruminating calves. DON and its acetylated metabolites were mainly metabolized to DON-3-glucuronide, however, also small amounts of DON-15-glucuronide were detected in urine. DON degradation to deepoxy-DON (DOM-1) was only observed to a relevant extent in ruminating calves. Consequently, toxicity of DON in calves is closely related to roughage provision and the associated stage of ruminal development.
Databáze: MEDLINE
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