Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryopreservation.

Autor:	Al-Otaibi NAS; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.; King Abdulaziz City for Science and Technology Kingdom of Saudi Arabia, PO, Riyadh, Saudi Arabia., Cassoli JS; Faculty of Medicine, Federal University of Tocantins (UFT), Palmas, Brazil., Slater NKH; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Rahmoune H; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK. hr288@cam.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1916, pp. 239-247.
DOI:	10.1007/978-1-4939-8994-2_23
Abstrakt:	The use of human leukemic (HL)-60 cells is important for studies of acute myeloid leukemia (AML) and as a model system for investigating how specific types of blood cells are formed during the process of hematopoiesis. Here, we present a protocol for growth of HL-60 cells along with molecular and functional profiles associated with their cryostorage. We also elucidate the effects of these procedures on cell viability and functions. This method can be used to provide biomarkers as readouts for testing the efficacy and/or toxicity of novel compounds in AML research as well as in a number of other experimental manipulations.
Databáze:	MEDLINE
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