Cytotoxicity and selectiveness of Brazilian Piper species towards oral carcinoma cells.

Autor: Macedo AL; Department of Organic Chemistry, Chemistry Institute, Fluminense Federal University, Niteroi, Brazil., da Silva DPD; Basic Science Department, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil., Moreira DL; Natural Products Department, Institute of Pharmaceutical Technology, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., de Queiroz LN; Basic Science Department, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil., Vasconcelos TRA; Department of Organic Chemistry, Chemistry Institute, Fluminense Federal University, Niteroi, Brazil., Araujo GF; Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil., Kaplan MAC; Institute of Research in Natural Products, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Pereira SSC; Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil., de Almeida ECP; Basic Science Department, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil., Valverde AL; Department of Organic Chemistry, Chemistry Institute, Fluminense Federal University, Niteroi, Brazil. Electronic address: alessandravalverde@id.uff.br., Robbs BK; Basic Science Department, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil; Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil; Postgraduate Program in Applied Science for Health Products, Faculty of Pharmacy, Fluminense Federal University, Niteroi, Brazil. Electronic address: brunokr@id.uff.br.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2019 Feb; Vol. 110, pp. 342-352. Date of Electronic Publication: 2018 Dec 04.
DOI: 10.1016/j.biopha.2018.11.129
Abstrakt: Background: Oral squamous cell carcinoma (OSCC) is one of the ten most common types of cancer worldwide. Plants of the genusPiper are used in traditional medicine to treat cancer, and they have a vast diversity of phytochemicals with cytotoxic potential. Purpose and Study Design: In this work, we analyzed the cytotoxic and selective potential of extracts and semipurified fractions of Piper mollicomum (PM), Piper truncatum (PT), Piper cernuum (PC), Piper arboreum (PA), and Piper cabralanum (PCa) using three different OSCC cell lines (SCC4, SCC9 and SCC25), and we measured their in vivo toxicities and conducted chemical analyses of their active fractions.
Results: The dichloromethane fractions of the crude methanolic extracts of the leaves of PM(-L-D), PC(-L-D) and PCa(-L-D) exhibited notable IC 50 values of 94.2, 47.2 and 47.5 μg/mL, respectively, and all three of these extracts were more active than carboplatin (172.3 μg/mL). The most selective fraction was PC-L-D, which exhibited SI > 4.5; less than 5% hemolysis; and no significant alterations in in vivo acute toxicology. The major constituents in active fractions were lignans (PC-L-D and PCa-L-D) and chromenes (PM-L-D).
Conclusion: PC-L-D demonstrated great potential for further development as an anticancer drug and could be the key to developing more effective and less toxic therapies against oral cancer.
(Copyright © 2018 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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